Western blot abreview for Anti-MBD2 antibody

Average
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Prostate (ventrale lobe))
Loading amount
5 µg
Specification
Prostate (ventrale lobe)
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1% in PBS 1X

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
Supersignal West pico Chemiluminsecent
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~43 kDa

Additional data

Additional Notes
Works on rat prostate (see WB protein gradient) but not that good on rat testis probably due to presence of other MBD isoforms, not tested on other rat tissues.
Lysis buffer : Tris 25 mM, SDS 0,1%, Protease Inhibitor Cocktail 1%
Transfer buffer : Tris 25mM, Glycine 185mM, Methanol 20%

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Submitted Jun 22 2007

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