Overview

  • Product name

  • Description

    Goat polyclonal to MBD2
  • Host species

    Goat
  • Specificity

    Expected to recognize isoform 1 only.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    RNDPLNQNKGKPDLN

    , corresponding to internal sequence amino acids 226-240 of Human MBD2

  • Positive control

    • Jurkat cell lysate Human colon

Properties

Applications

Our Abpromise guarantee covers the use of ab58241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.

Target

  • Function

    Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • Tissue specificity

    Highly expressed in brain, heart, kidney, stomach, testis and placenta.
  • Sequence similarities

    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization

    Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • Mbd2 antibody
    • MBD2_HUMAN antibody
    • MBD2a antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl-CpG-binding domain protein 2 antibody
    • Methyl-CpG-binding protein MBD2 antibody
    • NY CO 41 antibody
    see all

Images

  • ab58241 at 2.5 µg/ml staining MBD2 in Human skin by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed by AP-staining.

  • ab58241 at 2.5 µg/ml staining MBD2 in Human adrenal gland by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed by AP-staining.

  • Anti-MBD2 antibody (ab58241) at 0.3 µg/ml + Jurkat cell lysate at 35 µg

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Detected by chemiluminescence.
  • ab58241 at 2.5 µg/ml staining MBD2 in Human colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed by AP-staining.

References

This product has been referenced in:

  • Müller I  et al. Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications. BMC Cancer 10:297 (2010). WB ; Mouse . Read more (PubMed: 20565761) »
  • Mastroeni D  et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 31:2025-37 (2010). IHC-Fr ; Human . Read more (PubMed: 19117641) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Xenopus egg extract)
Specification
Xenopus egg extract
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 08 2011

Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
25 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 09 2011

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