Key features and details
- Rabbit polyclonal to MBD3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MBD3 antibody
See all MBD3 primary antibodies
DescriptionRabbit polyclonal to MBD3
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide aa 230-265. The exact sequence is proprietary. NP_003917.1
Database link: O95983
- IP: HeLa whole cell lysate.
ab245665 has not performed satisfactorily when used for WB of MBD3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MBD3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245665 was affinity purified using an epitope specific to PP1C gamma immobilized on solid support.
Our Abpromise guarantee covers the use of ab245665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionDoes not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
Cellular localizationNucleus. Nuclear, in discrete foci.
- Information by UniProt
- AI181826 antibody
- AU019209 antibody
- MBD 3 antibody
MBD3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245665 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245665 at 0.4 µg/ml.
Lane 1: ab245665 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245665 has not yet been referenced specifically in any publications.