Overview

  • Product name

  • Description

    Rabbit polyclonal to MBD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide aa 230-265. The exact sequence is proprietary. NP_003917.1
    Database link: O95983

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    ab245665 has not performed satisfactorily when used for WB of MBD3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MBD3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245665 was affinity purified using an epitope specific to PP1C gamma immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
    • Sequence similarities

      Contains 1 MBD (methyl-CpG-binding) domain.
    • Cellular localization

      Nucleus. Nuclear, in discrete foci.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI181826 antibody
      • AU019209 antibody
      • MBD 3 antibody
      • Mbd3 antibody
      • MBD3: methyl CpG binding domain protein 3 antibody
      • MBD3_HUMAN antibody
      • Methyl CpG binding domain protein 3 antibody
      • Methyl CpG binding protein MBD3 antibody
      • Methyl-CpG-binding domain protein 3 antibody
      • Methyl-CpG-binding protein MBD3 antibody
      see all

    Images

    • MBD3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245665 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245665 at 0.4 µg/ml.

      Lane 1: ab245665 IP in HeLa whole cell lysate.
      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 minutes.

      Lysates prepared in NETN buffer.

    References

    ab245665 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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