Product nameAnti-MBD3 antibody
See all MBD3 primary antibodies
DescriptionRabbit polyclonal to MBD3
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, OrangutanDoes not react with: Mouse
- HeLa or 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionDoes not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
Cellular localizationNucleus. Nuclear, in discrete foci.
- Information by UniProt
- AI181826 antibody
- AU019209 antibody
- MBD 3 antibody
All lanes : Anti-MBD3 antibody (ab91458) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91458.
20% of the immunoprecipitate was loaded per lane, and probed with ab91458 at 1µg/ml (lane 1) or a control IgG (lane2).
Detection: chemoluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Shimbo T et al. High-quality ChIP-seq analysis of MBD3 in human breast cancer cells. Genom Data 7:173-4 (2016). ChIP . Read more (PubMed: 26981404) »
- Shimbo T et al. MBD3 localizes at promoters, gene bodies and enhancers of active genes. PLoS Genet 9:e1004028 (2013). ChIP ; Human . Read more (PubMed: 24385926) »