Anti-MBD4/MED1 antibody (ab224809)
Key features and details
- Rabbit polyclonal to MBD4/MED1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MBD4/MED1 antibody
See all MBD4/MED1 primary antibodies -
Description
Rabbit polyclonal to MBD4/MED1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MBD4/MED1 aa 1-50. The exact sequence is proprietary. (NP_003916.1).
Database link: O95243 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab224809 was affinity purified using an epitope specific to MBD4/MED1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. -
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8930 Human
- Omim: 603574 Human
- SwissProt: O95243 Human
- Unigene: 35947 Human
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Alternative names
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
see all
Images
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All lanes : Anti-MBD4/MED1 antibody (ab224809) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes -
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MBD4/MED1 using ab224809 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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MBD4/MED1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224809 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224809 at 0.1 µg/ml.
Lane 1: ab224809 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224809 has been referenced in 1 publication.
- Chabot T et al. Cytidine analogs are synthetic lethal with base excision repair default due to MBD4 deficiency. NPJ Precis Oncol 6:81 (2022). PubMed: 36323843