Anti-MBD4/MED1 antibody (ab84754)
Key features and details
- Rabbit polyclonal to MBD4/MED1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MBD4/MED1 antibody
See all MBD4/MED1 primary antibodies -
Description
Rabbit polyclonal to MBD4/MED1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to Human MBD4/MED1 aa 1-50 (N terminal).
Database link: NP_003916.1 -
Positive control
- Human Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma and Testicular Seminoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab84754 was affinity purified using an epitope specific to MBD4/MED1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.
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Notes |
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IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining. |
Target
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Function
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. -
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8930 Human
- Omim: 603574 Human
- SwissProt: O95243 Human
- Unigene: 35947 Human
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Alternative names
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
see all
Images
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ab84754 at 1/250 dilution staining MBD4/MED1 in Human testicular seminoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
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ab84754 at 1/250 dilution staining MBD4/MED1 in Human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab84754 has been referenced in 2 publications.
- Tanakaya K et al. A germline MBD4 mutation was identified in a patient with colorectal oligopolyposis and early-onset cancer: A case report. Oncol Rep 42:1133-1140 (2019). PubMed: 31322271
- Hata K et al. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey. J Neurosci 33:19704-14 (2013). Macaque monkey . PubMed: 24336734