Key features and details
- Rabbit polyclonal to MBD4/MED1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MBD4/MED1 antibody
See all MBD4/MED1 primary antibodies
DescriptionRabbit polyclonal to MBD4/MED1
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MBD4/MED1 aa 1-50. The exact sequence is proprietary. (NP_003916.1).
Database link: O95243
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
This product was previously labelled as MBD4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224809 was affinity purified using an epitope specific to MBD4/MED1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
- Information by UniProt
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
All lanes : Anti-MBD4/MED1 antibody (ab224809) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 3 minutes
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for MBD4/MED1 using ab224809 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
MBD4/MED1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224809 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224809 at 0.1 µg/ml.
Lane 1: ab224809 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224809 has not yet been referenced specifically in any publications.