Overview

  • Product name

  • Description

    Rabbit polyclonal to MBD4/MED1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human MBD4/MED1 (internal sequence). The exact sequence is proprietary.
    Database link: O95243

  • Positive control

    • WB: MBD4/MED1 transfected HEK-293T whole cell extract. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as MBD4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • Sequence similarities

    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 N(4) ethenocytosine glycosylase antibody
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
    • G/T mismatch glycosylase antibody
    • G/U mismatch glycosylase antibody
    • MBD 4 antibody
    • MBD4 antibody
    • MBD4_HUMAN antibody
    • MED 1 antibody
    • MED1 antibody
    • Methyl CpG binding domain protein 4 antibody
    • Methyl CpG binding endonuclease 1 antibody
    • Methyl CpG binding protein MBD4 antibody
    • Methyl-CpG-binding domain protein 4 antibody
    • Methyl-CpG-binding endonuclease 1 antibody
    • Methyl-CpG-binding protein MBD4 antibody
    • Mismatch specific DNA N glycosylase antibody
    • Mismatch-specific DNA N-glycosylase antibody
    • Putative methyl CpG binding protein antibody
    see all

Images

  • All lanes : Anti-MBD4/MED1 antibody (ab227625) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : MBD4/MED1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 66 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MBD4/MED1 (green) using ab227625 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab227625 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227625.
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