Product nameAnti-MBD4/MED1 antibody
See all MBD4/MED1 primary antibodies
DescriptionRabbit polyclonal to MBD4/MED1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant fragment within Human MBD4/MED1 (internal sequence). The exact sequence is proprietary.
Database link: O95243
- WB: MBD4/MED1 transfected HEK-293T whole cell extract. ICC/IF: HeLa cells.
This product was previously labelled as MBD4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
- Information by UniProt
- 3 N(4) ethenocytosine glycosylase antibody
- G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
- G/T mismatch glycosylase antibody
All lanes : Anti-MBD4/MED1 antibody (ab227625) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : MBD4/MED1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 66 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MBD4/MED1 (green) using ab227625 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
ab227625 has not yet been referenced specifically in any publications.