Overview

  • Product name

    Anti-MBD4/MED1 antibody - C-terminal
    See all MBD4/MED1 primary antibodies
  • Description

    Rabbit polyclonal to MBD4/MED1 - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human MBD4/MED1 aa 566-580 (C terminal).
    Sequence:

    YHDWLWENHEKLSLS


    Database link: O95243

  • Positive control

    • Rat brain and kidney tissue lysates; A549, HeLa and MCF-7 cell lysates; Recombinant Human MBD4/MED1 protein (aa400-580).
  • General notes

     This product was previously labelled as MBD4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 66 kDa.

The detection limit for MBD4 is approximately 1ng/lane under reducing conditions.

Target

  • Function

    Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • Sequence similarities

    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 N(4) ethenocytosine glycosylase antibody
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
    • G/T mismatch glycosylase antibody
    • G/U mismatch glycosylase antibody
    • MBD 4 antibody
    • MBD4 antibody
    • MBD4_HUMAN antibody
    • MED 1 antibody
    • MED1 antibody
    • Methyl CpG binding domain protein 4 antibody
    • Methyl CpG binding endonuclease 1 antibody
    • Methyl CpG binding protein MBD4 antibody
    • Methyl-CpG-binding domain protein 4 antibody
    • Methyl-CpG-binding endonuclease 1 antibody
    • Methyl-CpG-binding protein MBD4 antibody
    • Mismatch specific DNA N glycosylase antibody
    • Mismatch-specific DNA N-glycosylase antibody
    • Putative methyl CpG binding protein antibody
    see all

Images

  • All lanes : Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate

    Predicted band size: 66 kDa

  • All lanes : Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat kidney tissue lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : MCF-7 cell lysate

    Predicted band size: 66 kDa

  • All lanes : Anti-MBD4/MED1 antibody - C-terminal (ab191037) at 0.5 µg/ml

    Lane 1 : Recombinant Human MBD4/MED1 protein (aa400-580) at 0.01 µg
    Lane 2 : Recombinant Human MBD4/MED1 protein (aa400-580) at 0.005 µg
    Lane 3 : Recombinant Human MBD4/MED1 protein (aa400-580) at 0.0025 µg

    Predicted band size: 66 kDa



    E.coli derived -recombinant Human MBD4/MED1, 39.7 kDa (162aa tag + Q400-S580).

References

ab191037 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191037.
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