Overview

  • Product name

  • Description

    Rabbit polyclonal to MBD6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human MBD6 aa 624-715.
    Sequence:

    SFLPLLALGPTAGDGEGSAEGAGGPSGEPFSGLGDLSPLLFPPLSAPPTL IALNSALLAATLDPPSGTPPQPCVLSAPQPGPPTSSVTTATT


    Database link: Q96DN6

  • Positive control

    • Human adrenal gland tissue; U-2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab204403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling MBD6 with ab204403 at 1/50 dilution.

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling MBD6 with ab204403 at 4 µg/mL (green).

References

ab204403 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Sep 28 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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