Anti-MBD6 antibody (ab204403)
Key features and details
- Rabbit polyclonal to MBD6
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MBD6 antibody -
Description
Rabbit polyclonal to MBD6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human MBD6 aa 600-750.
Database link: Q96DN6 -
Positive control
- Human adrenal gland tissue; U-2OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro). -
Sequence similarities
Contains 1 MBD (methyl-CpG-binding) domain. -
Domain
The MBD domain is necessary for chromocentric localization. -
Cellular localization
Nucleus. Chromosome. Associated with pericentric heterochromatin in about 25% of the cells. - Information by UniProt
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Database links
- Entrez Gene: 114785 Human
- Entrez Gene: 110962 Mouse
- Entrez Gene: 362892 Rat
- SwissProt: Q96DN6 Human
- SwissProt: Q3TY92 Mouse
- Unigene: 524523 Human
- Unigene: 372826 Mouse
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Alternative names
- MBD6 antibody
- MBD6_HUMAN antibody
- Methyl-CpG-binding domain protein 6 antibody
- Methyl-CpG-binding protein MBD6 antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab204403 has been referenced in 1 publication.
- Wang XH et al. Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Nat Commun 12:3428 (2021). PubMed: 34103526