Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13952] to MBIP
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Product nameAnti-MBIP antibody [EPR13952]
See all MBIP primary antibodies
DescriptionRabbit monoclonal [EPR13952] to MBIP
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
within Human MBIP aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NS73
- 293T, HepG2, HeLa and Caco-2 cell lysates. HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 39 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionInhibits the MAP3K12 activity to induce the activation of the JNK/SAPK pathway. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Tissue specificityUbiquitous. High expression seen in the heart and lung.
Cellular localizationNucleus. Cytoplasm. Shows a cytoplasmic localization when coexpressed with MAP3K12.
- Information by UniProt
- MAP3K12 binding inhibitory protein 1 antibody
- MAP3K12-binding inhibitory protein 1 antibody
- MAPK upstream kinase binding inhibitory protein antibody
All lanes : Anti-MBIP antibody [EPR13952] (ab181040) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Hela cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
Immunofluorescent staining of HepG2 cells labeling MBIP with ab181040 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using ab181040 at 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab181040 has not yet been referenced specifically in any publications.