Overview

  • Product name

    Anti-MBNL1 antibody [EPR4565]
    See all MBNL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4565] to MBNL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MBNL1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, and A673 cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as Muscleblind-like 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4565
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
    • Tissue specificity

      Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in disease

      Plays a role in the pathogenesis of dystrophia myotonica type 1 (DM1) [MIM:160900]. A muscular disorder characterized by myotonia, muscle wasting in the distal extremities, cataract, hypogonadism, defective endocrine functions, male baldness and cardiac arrhythmias. Note=In muscle cells from DM1 patients, MBNL1 is sequestered by DMPK RNAs containing CUG triplet repeat expansions. MBNL1 binding is proportional to repeat length consistent with the direct correlation between the length of repeat expansion and disease severity.
    • Sequence similarities

      Belongs to the muscleblind family.
      Contains 4 C3H1-type zinc fingers.
    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasmic granule. Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress. Localized in the cytoplasm of multinucleated myotubes. Colocalizes with nuclear foci of retained expanded-repeat transcripts in myotubes from patients affected by myotonic dystrophy.
    • Information by UniProt
    • Database links

    • Alternative names

      • EXP antibody
      • EXP35 antibody
      • EXP40 antibody
      • EXP42 antibody
      • KIAA0428 antibody
      • MBNL antibody
      • MBNL protein antibody
      • MBNL1 antibody
      • MBNL1_HUMAN antibody
      • Muscleblind 41kD isoform antibody
      • Muscleblind like antibody
      • Muscleblind like protein 1 antibody
      • Muscleblind like splicing regulator 1 antibody
      • Muscleblind-like protein 1 antibody
      • Triplet expansion RNA binding protein antibody
      • Triplet-expansion RNA-binding protein antibody
      see all

    Images

    • All lanes : Anti-MBNL1 antibody [EPR4565] (ab108519) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa

    References

    This product has been referenced in:

    • Idris M  et al. The MBNL/CELF Splicing Factors Regulate Cytosolic Sulfotransferase 4A1 Protein Expression during Cell Differentiation. Drug Metab Dispos 47:314-319 (2019). Read more (PubMed: 30606728) »
    • Liu H  et al. Alternative splicing analysis in human monocytes and macrophages reveals MBNL1 as major regulator. Nucleic Acids Res 46:6069-6086 (2018). Read more (PubMed: 29771377) »
    See all 6 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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