Recombinant Anti-MBNL3 antibody [EPR16337] (ab197590)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16337] to MBNL3
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MBNL3 antibody [EPR16337]
See all MBNL3 primary antibodies -
Description
Rabbit monoclonal [EPR16337] to MBNL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB; Human placenta lysate. ICC/IF: JAR cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16337 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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ICC/IF |
1/800.
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Notes |
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WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
1/800. |
Target
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Function
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction. -
Tissue specificity
Highly expressed in the placenta. -
Sequence similarities
Belongs to the muscleblind family.
Contains 4 C3H1-type zinc fingers. -
Cellular localization
Nucleus. Cytoplasm. Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts. - Information by UniProt
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Database links
- Entrez Gene: 55796 Human
- Omim: 300413 Human
- SwissProt: Q9NUK0 Human
- Unigene: 105134 Human
- Unigene: 596347 Human
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Alternative names
- A530038J18Rik antibody
- AI661274 antibody
- CHCR antibody
see all
Images
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Anti-MBNL3 antibody [EPR16337] (ab197590) at 1/1000 dilution + Human placenta lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
The expression is consistent with what has been described in the literature (PMID:)11929853.
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ab197590 staining MBNL3 in JAR (human choriocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab197590 has been referenced in 1 publication.
- Yu Z et al. LncRNA SBF2-AS1 affects the radiosensitivity of non-small cell lung cancer via modulating microRNA-302a/MBNL3 axis. Cell Cycle 19:300-316 (2020). PubMed: 31928130