Key features and details
- Rabbit polyclonal to MBOAT2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MBOAT2 antibody
DescriptionRabbit polyclonal to MBOAT2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human colon tissue; RT-4 and U-251 MG cell lysates; Human Liver lysate
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionAcyltransferase which mediates the conversion of lysophosphatidylethanolamine (1-acyl-sn-glycero-3-phosphoethanolamine or LPE) into phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine or PE) (LPEAT activity). Catalyzes also the acylation of lysophosphatidic acid (LPA) into phosphatidic acid (PA) (LPAAT activity). Has also a very weak lysophosphatidylcholine acyltransferase (LPCAT activity). Prefers oleoyl-CoA as the acyl donor. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.
Tissue specificityExpressed in neutrophils.
PathwayLipid metabolism; phospholipid metabolism.
Sequence similaritiesBelongs to the membrane-bound acyltransferase family.
- Information by UniProt
- 1-acylglycerophosphate O-acyltransferase antibody
- 1-acylglycerophosphoethanolamine O-acyltransferase antibody
- LPAAT antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121453 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121453, at 1/1500 dilution, staining MBOAT2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
All lanes : Anti-MBOAT2 antibody (ab121453) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma lysate
Lane 5 : Human Liver lysate
Lane 6 : Human Tonsil lysate
ab121453 has not yet been referenced specifically in any publications.