Overview

  • Product name

  • Description

    Rabbit polyclonal to MC-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Hamster, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human MC-2 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125889)

  • Positive control

    • This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.
  • General notes

    Previously labelled as MC5 Receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab87470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).

Target

Images

  • Lane 1 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)
    Lane 2 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)

    All lanes : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Lane 2 : Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    MC-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk (3%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab87470 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab87470.
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