Product nameAnti-MC-2 antibody
See all MC-2 primary antibodies
DescriptionRabbit polyclonal to MC-2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Hamster, Chimpanzee, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human MC-2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.
Previously labelled as MC5 Receptor.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).|
FunctionReceptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
Tissue specificityExpressed in the brain but not in the melanoma cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- MC-2 antibody
- MC2 antibody
- MC5 R antibody
Lane 1 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 5% BSA)
Lane 2 : Anti-MC-2 antibody (ab87470) at 1 µg/ml (Blocked in 3% MILK)
All lanes : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Lane 2 : Recombinant rat MCP1 protein (ab9780) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
MC-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam recommends using milk (3%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab87470 has not yet been referenced specifically in any publications.