Product nameAnti-MC-2 antibody [EPR8386]
See all MC-2 primary antibodies
DescriptionRabbit monoclonal [EPR8386] to MC-2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MC-2 aa 1-100 (extracellular). The exact sequence is proprietary.
- HeLa, K562, U-87 MG, HepG2 and Human fetal brain lysates; Human brain tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.35 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
Tissue specificityExpressed in the brain but not in the melanoma cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- MC-2 antibody
- MC2 antibody
- MC5 R antibody
All lanes : Anti-MC-2 antibody [EPR8386] (ab133656) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling MC-2 with ab133656 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD