Anti-MC1-R antibody [578CT6.2.3] (ab230675)
Key features and details
- Mouse monoclonal [578CT6.2.3] to MC1-R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-MC1-R antibody [578CT6.2.3]
See all MC1-R primary antibodies -
Description
Mouse monoclonal [578CT6.2.3] to MC1-R -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cat, Chimpanzee, Gorilla -
Immunogen
Synthetic peptide within Human MC1-R aa 205-232 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:VLYVHMLARACQHAQGIARLHKRQRPVH
Database link: Q01726 -
Positive control
- WB: A-375 cell lysate. IHC-P: Human brain cortex tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purification notes
Euglobulin precipitation from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
578CT6.2.3 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab230675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/100 - 1/250. Predicted molecular weight: 35 kDa. | |
IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. -
Tissue specificity
Melanocytes and corticoadrenal tissue. -
Involvement in disease
Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 493917 Cat
- Entrez Gene: 493980 Chimpanzee
- Entrez Gene: 101131674 Gorilla
- Entrez Gene: 4157 Human
- Omim: 155555 Human
- SwissProt: Q864K9 Gorilla
- SwissProt: Q01726 Human
- Unigene: 513829 Human
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Alternative names
- CMM5 antibody
- MC1 Receptor antibody
- MC1-R antibody
see all
Images
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Anti-MC1-R antibody [578CT6.2.3] (ab230675) at 1/100 dilution + A-375 (human malignant melanoma cell line) cell lysate at 35 µg
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [578CT6.2.3] (ab230675)
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for MC1-R using ab230675 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab230675 has not yet been referenced specifically in any publications.