Anti-MC1-R antibody [578CT6.2.3] (ab230675)

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Western blot - Anti-MC1-R antibody [578CT6.2.3] (ab230675)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [578CT6.2.3] (ab230675)

Key features and details

  • Mouse monoclonal [578CT6.2.3] to MC1-R
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-MC1-R antibody [578CT6.2.3]
    See all MC1-R primary antibodies

  • Description

    Mouse monoclonal [578CT6.2.3] to MC1-R

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cat, Chimpanzee, Gorilla

  • Immunogen

    Synthetic peptide within Human MC1-R aa 205-232 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    VLYVHMLARACQHAQGIARLHKRQRPVH


    Database link: Q01726
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: A-375 cell lysate. IHC-P: Human brain cortex tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab230675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 35 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

  • Tissue specificity

    Melanocytes and corticoadrenal tissue.

  • Involvement in disease

    Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CMM5 antibody
      • MC1 Receptor antibody
      • MC1-R antibody
      • MC1R antibody
      • Melanocortin 1 receptor antibody
      • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
      • Melanocortin receptor 1 antibody
      • Melanocyte-stimulating hormone receptor antibody
      • Melanotropin receptor antibody
      • MSH-R antibody
      • MSHR antibody
      • MSHR_HUMAN antibody
      • SHEP2 antibody
      see all

    Images

    • Western blot - Anti-MC1-R antibody [578CT6.2.3] (ab230675)
      Western blot - Anti-MC1-R antibody [578CT6.2.3] (ab230675)
      Anti-MC1-R antibody [578CT6.2.3] (ab230675) at 1/100 dilution + A-375 (human malignant melanoma cell line) cell lysate at 35 µg

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [578CT6.2.3] (ab230675)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [578CT6.2.3] (ab230675)

      Formalin-fixed, paraffin-embedded human brain cortex tissue stained for MC1-R using ab230675 at 1/100 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab230675? Please let us know so that we can cite the reference in this datasheet.

    ab230675 has not yet been referenced specifically in any publications.

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