Overview

  • Product name

  • Description

    Rabbit polyclonal to MC1-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment corresponding to Human MC1-R aa 1-37.
    Sequence:

    MAVQGSQRRLLGSLNSTPTAIPQLGLAANQTGARCLE


    Database link: Q01726

  • Positive control

    • WB: A-375 whole cell lysate. IHC-P: Human melanoma and brain tissues. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as MC1 Receptor. 

Properties

Applications

Our Abpromise guarantee covers the use of ab236734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.
WB 1/500 - 1/5000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity

    Melanocytes and corticoadrenal tissue.
  • Involvement in disease

    Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMM5 antibody
    • MC1 Receptor antibody
    • MC1-R antibody
    • MC1R antibody
    • Melanocortin 1 receptor antibody
    • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
    • Melanocortin receptor 1 antibody
    • Melanocyte-stimulating hormone receptor antibody
    • Melanotropin receptor antibody
    • MSH-R antibody
    • MSHR antibody
    • MSHR_HUMAN antibody
    • SHEP2 antibody
    see all

Images

  • Paraffin-embedded human melanoma tissue stained for MC1-R using ab236734 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Anti-MC1-R antibody (ab236734) at 1/500 dilution + A-375 (human malignant melanoma cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa

  • Paraffin-embedded human brain tissue stained for MC1-R using ab236734 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained forMC1-R (green) using ab236734 at 1/133 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG(H+L).

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.

References

ab236734 has not yet been referenced specifically in any publications.

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