Product nameAnti-MC1-R antibody [EPR6530]
See all MC1-R primary antibodies
DescriptionRabbit monoclonal [EPR6530] to MC1-R
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
(Peptide available as
- WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as MC1 Receptor.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.93 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionReceptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
Tissue specificityMelanocytes and corticoadrenal tissue.
Involvement in diseaseGenetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- CMM5 antibody
- MC1 Receptor antibody
- MC1-R antibody
All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : Human melanoma lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 35 kDa
ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Wang L et al. a-melanocyte stimulating hormone (a-MSH) promotes osteoblast differentiation of MC3T3-E1 cells. Eur J Pharmacol 844:1-8 (2019). Read more (PubMed: 30496742) »
- Chen S et al. Targeting MC1R depalmitoylation to prevent melanomagenesis in redheads. Nat Commun 10:877 (2019). Read more (PubMed: 30787281) »