Recombinant
RabMAb

Recombinant Anti-MC1-R antibody [EPR6530] (ab125031)

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Overview

  • Product name

    Anti-MC1-R antibody [EPR6530]
    See all MC1-R primary antibodies

  • Description

    Rabbit monoclonal [EPR6530] to MC1-R

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
    (Peptide available as ab195973)

  • Positive control

    • WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as MC1 Receptor. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

    • Tissue specificity

      Melanocytes and corticoadrenal tissue.

    • Involvement in disease

      Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CMM5 antibody
        • MC1 Receptor antibody
        • MC1-R antibody
        • MC1R antibody
        • Melanocortin 1 receptor antibody
        • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
        • Melanocortin receptor 1 antibody
        • Melanocyte-stimulating hormone receptor antibody
        • Melanotropin receptor antibody
        • MSH-R antibody
        • MSHR antibody
        • MSHR_HUMAN antibody
        • SHEP2 antibody
        see all

      Images

      • Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)
        Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)
        All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution

        Lane 1 : A375 cell lysate
        Lane 2 : Human melanoma lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

        Predicted band size: 35 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)

        ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Other - Anti-MC1-R antibody [EPR6530] (ab125031)
        Other - Anti-MC1-R antibody [EPR6530] (ab125031)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      See all 3 Publications for this product

      Publishing research using ab125031? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MC1-R antibody [EPR6530]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (skin)
      Antigen retrieval step
      None
      Permeabilization
      No
      Specification
      skin
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 12 2012

      Western blot abreview for Anti-MC1-R antibody [EPR6530]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (skin biopsy and cells)
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (5-16% gel)
      Loading amount
      20 µg
      Specification
      skin biopsy and cells
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 12 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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