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    mc1-r-antibody-epr6530-ab125031.pdf

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RecombinantRabMAb

Recombinant Anti-MC1-R antibody [EPR6530] (ab125031)

  • Datasheet
Reviews (2) Submit a question References (6)

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Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)
  • OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031)
  • Anti-MC1-R antibody [EPR6530] (ab125031)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6530] to MC1-R
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-MC1-R antibody [EPR6530]
    See all MC1-R primary antibodies
  • Description

    Rabbit monoclonal [EPR6530] to MC1-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
    (Peptide available as ab195973)

  • Positive control

    • WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.93 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6530
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Alternative Versions

    • Anti-MC1-R antibody [EPR6530] - BSA and Azide free (ab240017)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MC1-R protein (ab152515)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P (1)
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC or IP.

Target

  • Function

    Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity

    Melanocytes and corticoadrenal tissue.
  • Involvement in disease

    Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q01726 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4157 Human
    • Omim: 155555 Human
    • SwissProt: Q01726 Human
    • Unigene: 513829 Human
    • Alternative names

      • CMM5 antibody
      • MC1 Receptor antibody
      • MC1-R antibody
      • MC1R antibody
      • Melanocortin 1 receptor antibody
      • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
      • Melanocortin receptor 1 antibody
      • Melanocyte-stimulating hormone receptor antibody
      • Melanotropin receptor antibody
      • MSH-R antibody
      • MSHR antibody
      • MSHR_HUMAN antibody
      • SHEP2 antibody
      see all

    Images

    • Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)
      Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)
      All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution

      Lane 1 : A375 cell lysate
      Lane 2 : Human melanoma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)

      ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031)
      OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-MC1-R antibody [EPR6530] (ab125031)
      Anti-MC1-R antibody [EPR6530] (ab125031)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (6)

    Publishing research using ab125031? Please let us know so that we can cite the reference in this datasheet.

    ab125031 has been referenced in 6 publications.

    • Dong D  et al. NB-UVB Induces Melanocytic Differentiation of Human Hair Follicle Neural Crest Stem Cells. Ann Dermatol 32:289-297 (2020). PubMed: 33911756
    • Wang L  et al. a-melanocyte stimulating hormone (a-MSH) promotes osteoblast differentiation of MC3T3-E1 cells. Eur J Pharmacol 844:1-8 (2019). PubMed: 30496742
    • Chen S  et al. Targeting MC1R depalmitoylation to prevent melanomagenesis in redheads. Nat Commun 10:877 (2019). PubMed: 30787281
    • Li M  et al. Enhancing the Efficacy of Melanocortin 1 Receptor-Targeted Radiotherapy by Pharmacologically Upregulating the Receptor in Metastatic Melanoma. Mol Pharm 16:3904-3915 (2019). PubMed: 31318566
    • Chen F  et al. Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging. ACS Appl Mater Interfaces 10:4379-4393 (2018). PubMed: 29058865
    • Lantang AM  et al. Expression of melanocortin receptors in human endometrium. Hum Reprod 30:2404-10 (2015). PubMed: 26223677

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MC1-R antibody [EPR6530]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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