Recombinant Anti-MC1-R antibody [EPR6530] (ab125031)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6530] to MC1-R
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MC1-R antibody [EPR6530]
See all MC1-R primary antibodies -
Description
Rabbit monoclonal [EPR6530] to MC1-R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
(Peptide available asab195973) -
Positive control
- WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as MC1 Receptor.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.93 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6530 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab125031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 35 kDa. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. -
Tissue specificity
Melanocytes and corticoadrenal tissue. -
Involvement in disease
Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4157 Human
- Omim: 155555 Human
- SwissProt: Q01726 Human
- Unigene: 513829 Human
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Alternative names
- CMM5 antibody
- MC1 Receptor antibody
- MC1-R antibody
see all
Images
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![Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139174-anti-mc1-r-antibody-epr6530-western-blot.jpg)
Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : Human melanoma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139173-anti-mc1-r-antibody-epr6530-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139172-anti-mc1-r-antibody-epr6530.jpg)
OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031) -
![Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-4-benefits-of-recombinant-antibodies.png)
Anti-MC1-R antibody [EPR6530] (ab125031)
Protocols
Datasheets and documents
References (4)
ab125031 has been referenced in 4 publications.
- Wang L et al. a-melanocyte stimulating hormone (a-MSH) promotes osteoblast differentiation of MC3T3-E1 cells. Eur J Pharmacol 844:1-8 (2019). PubMed: 30496742
- Chen S et al. Targeting MC1R depalmitoylation to prevent melanomagenesis in redheads. Nat Commun 10:877 (2019). PubMed: 30787281
- Chen F et al. Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging. ACS Appl Mater Interfaces 10:4379-4393 (2018). PubMed: 29058865
- Lantang AM et al. Expression of melanocortin receptors in human endometrium. Hum Reprod 30:2404-10 (2015). PubMed: 26223677
