Recombinant Anti-MC1-R antibody [EPR6530] (ab125031)
![Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139174-anti-mc1-r-antibody-epr6530-western-blot.jpg "Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6530] to MC1-R
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MC1-R antibody [EPR6530]
See all MC1-R primary antibodies -
Description
Rabbit monoclonal [EPR6530] to MC1-R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
(Peptide available asab195973) -
Positive control
- WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.93 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6530 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
|
| IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt,ICC or IP.
Target
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Function
Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. -
Tissue specificity
Melanocytes and corticoadrenal tissue. -
Involvement in disease
Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4157 Human
- Omim: 155555 Human
- SwissProt: Q01726 Human
- Unigene: 513829 Human
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Alternative names
- CMM5 antibody
- MC1 Receptor antibody
- MC1-R antibody
see all
Images
-
Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : Human melanoma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139173-anti-mc1-r-antibody-epr6530-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139172-anti-mc1-r-antibody-epr6530.jpg)
OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031) -
![Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-4-benefits-of-recombinant-antibodies.png)
Anti-MC1-R antibody [EPR6530] (ab125031)
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab125031 has been referenced in 6 publications.
- Dong D et al. NB-UVB Induces Melanocytic Differentiation of Human Hair Follicle Neural Crest Stem Cells. Ann Dermatol 32:289-297 (2020). PubMed: 33911756
- Wang L et al. a-melanocyte stimulating hormone (a-MSH) promotes osteoblast differentiation of MC3T3-E1 cells. Eur J Pharmacol 844:1-8 (2019). PubMed: 30496742
- Chen S et al. Targeting MC1R depalmitoylation to prevent melanomagenesis in redheads. Nat Commun 10:877 (2019). PubMed: 30787281
- Li M et al. Enhancing the Efficacy of Melanocortin 1 Receptor-Targeted Radiotherapy by Pharmacologically Upregulating the Receptor in Metastatic Melanoma. Mol Pharm 16:3904-3915 (2019). PubMed: 31318566
- Chen F et al. Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging. ACS Appl Mater Interfaces 10:4379-4393 (2018). PubMed: 29058865
- Lantang AM et al. Expression of melanocortin receptors in human endometrium. Hum Reprod 30:2404-10 (2015). PubMed: 26223677
