Overview

  • Product name

    Anti-MC1-R antibody [EPR6530]
    See all MC1-R primary antibodies
  • Description

    Rabbit monoclonal [EPR6530] to MC1-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
    (Peptide available as ab195973)

  • Positive control

    • WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as MC1 Receptor. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
    • Tissue specificity

      Melanocytes and corticoadrenal tissue.
    • Involvement in disease

      Genetic variations in MC1R are a cause of susceptibility to cutaneous malignant melanoma type 5 (CMM5) [MIM:613099]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CMM5 antibody
      • MC1 Receptor antibody
      • MC1-R antibody
      • MC1R antibody
      • Melanocortin 1 receptor antibody
      • Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) antibody
      • Melanocortin receptor 1 antibody
      • Melanocyte-stimulating hormone receptor antibody
      • Melanotropin receptor antibody
      • MSH-R antibody
      • MSHR antibody
      • MSHR_HUMAN antibody
      • SHEP2 antibody
      see all

    Images

    • All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution

      Lane 1 : A375 cell lysate
      Lane 2 : Human melanoma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (skin biopsy and cells)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gel)
    Loading amount
    20 µg
    Specification
    skin biopsy and cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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