Overview

  • Product name
  • Description
    Rabbit polyclonal to MC3-R
  • Host species
    Rabbit
  • Specificity
    The antibody does not cross reacts with any other MC receptors.
  • Tested applications
    Suitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to MC3-R (N terminal).

  • General notes

    Previously labelled as MC3 Receptor. 

Properties

Applications

Our Abpromise guarantee covers the use of ab21228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Receptor for MSH (alpha, beta and gamma) and ACTH. This receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity
    Brain, placental, and gut tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMIQ9 antibody
    • MC3 antibody
    • MC3 R antibody
    • MC3 Receptor antibody
    • MC3-R antibody
    • Mc3r antibody
    • MC3R_HUMAN antibody
    • Melanocortin 3 receptor antibody
    • Melanocortin receptor 3 antibody
    • OB20 antibody
    • Obesity quantitative trait locus antibody
    • OQTL antibody
    see all

References

ab21228 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

My colleague is currently out of the office.
The source of the antibodyconfirmed that this antibody should show a band at apparent MW of 45kDa provided the samples are processed correctly. They have seen dimerization of certain GPCR proteins upon repeated freezing and thawing cycles, this was apparent in Dopamine D2 receptor which dimerizes and appear as higher MW bands upon heating and thawing.
Theywould suggest not to heat the protein sample in SDS-PAGE sample buffer rather incubate at 37ºC for 30-45min and then apply on the gel.
The size of this protein varies by several kDa in different cell lines probably due to post-translational modifications.

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioningI would be pleased to provide a credit note or afree of charge replacement with an alternative anti-MC3 from this list: https://www.abcam.com/index.html?t=114723&pt=1 .

I hope this information is helpful, and I thank you for your cooperation.

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Answer

Thank you for your enquiry. Regarding ab21228, ab21229, and ab21231 (MC3 receptor antibodies), the immunogenic peptide sequence is proprietary information. However, we have performed a sequence alignment for you and here are the results: ab21228 will react with the human protein only. ab21229 reacts with Human and Rat and is predicted to cross-react with: Mouse, Elephas maximus (Asiatic elephant), Ovis aries (sheep), Bos taurus, Monkey, Canis familiaris, and Pan troglodytes. ab21231 reacts with Human, Rat, and Mouse and is predicted to cross-react with: Elephas maximus, Ovis aries (sheep), Bos taurus (bovine), Monkey, Canis familiaris, Sus scrofa (pig), and Pan troglodytes (Chimp). Based on this information, I suggest testing ab21231. I hope this helps. Please don't hesitate to contact us again if you have further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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