Anti-MC4-R antibody (ab106596)
Key features and details
- Rabbit polyclonal to MC4-R
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MC4-R antibody
See all MC4-R primary antibodies -
Description
Rabbit polyclonal to MC4-R -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human MC4-R (N terminal).
Database link: NP_005903 -
Positive control
- Rat brain tissue lysate; Human brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab106596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 2.5 µg/ml. | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 37 kDa. |
Target
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Function
Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase. -
Tissue specificity
Brain, placental, and gut tissues. -
Involvement in disease
Defects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4160 Human
- Entrez Gene: 17202 Mouse
- Entrez Gene: 25635 Rat
- Omim: 155541 Human
- SwissProt: P32245 Human
- SwissProt: P56450 Mouse
- SwissProt: P70596 Rat
- Unigene: 532833 Human
see all -
Alternative names
- MC4 Receptor antibody
- MC4-R antibody
- MC4R antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC4-R antibody (ab106596)
ab106596, at 2.5 µg/ml, staining MC4-R in Human brain tissue by Immunohistochemistry.
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Western blot - Anti-MC4-R antibody (ab106596)All lanes : Anti-MC4-R antibody (ab106596) at 1 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 37 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MC4-R antibody (ab106596)Immunofluorescence of MC4-R in Human Brain cells using ab106596 at 20 ug/ml.
Protocols
Datasheets and documents
References (0)
ab106596 has not yet been referenced specifically in any publications.
