Product nameAnti-MC4-R antibody
See all MC4-R primary antibodies
DescriptionRabbit polyclonal to MC4-R
Tested applicationsSuitable for: WB, IHC-P, ELISA, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4
Concentration information loading...
PurityProtein A purified
Primary antibody notesThis antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab24233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Mouse MC4-R peptide (ab78212). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein.|
|IHC-P||Use a concentration of 1 µg/ml.|
|ELISA||Use a concentration of 0.4 µg/ml.|
FunctionReceptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
Tissue specificityBrain, placental, and gut tissues.
Involvement in diseaseDefects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- MC4 Receptor antibody
- MC4-R antibody
- MC4R antibody
ab24233 staining MC4 Receptor in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/1000 in TPBS + normal goat serum) for 19 hours at 4°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
All lanes : Anti-MC4-R antibody (ab24233)
Lane 1 : Human cerebella cortex pellet.
Lane 2 : Human hippocampus homogenate.
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Additional bands at: 55 kDa (possible glycosylated form)
Immunoperoxidase staining of rat brain section with ab24233. Note the tadpole like staining of cortex neurons.
This product has been referenced in:
- Peter JC et al. A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration. J Pharmacol Exp Ther 333:478-90 (2010). WB, IP ; Human . Read more (PubMed: 20118207) »
- Chitravanshi VC et al. Microinjections of alpha-melanocyte stimulating hormone into the nucleus ambiguus of the rat elicit vagally mediated bradycardia. Am J Physiol Regul Integr Comp Physiol 296:R1402-11 (2009). IHC-FoFr ; Rat . Read more (PubMed: 19297540) »