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RecombinantRabMAb

Recombinant Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)

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Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)
  • Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6534(2)(ABC)] to MC4-R
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MC4-R antibody [EPR6534(2)(ABC)]
    See all MC4-R primary antibodies
  • Description

    Rabbit monoclonal [EPR6534(2)(ABC)] to MC4-R
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human MC4-R aa 1-100 (C terminal). The exact sequence is proprietary.
    Database link: P32245

  • Positive control

    • 293T; MCF7; JAR; SH-SY5Y; C6; RAW 264.7; PC12 and NIH 3T3 cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6534(2)(ABC)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Neuroscience
    • Neurology process
    • Metabolism
    • Neuroscience
    • Endocrine system
    • Hypothalamic pituitary adrenal axis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-MC4-R antibody [EPR6534(2)(ABC)] - BSA and Azide free (ab248980)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase.
    • Tissue specificity

      Brain, placental, and gut tissues.
    • Involvement in disease

      Defects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P32245 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4160 Human
      • Entrez Gene: 17202 Mouse
      • Entrez Gene: 25635 Rat
      • Omim: 155541 Human
      • SwissProt: P32245 Human
      • SwissProt: P56450 Mouse
      • SwissProt: P70596 Rat
      • Unigene: 532833 Human
      • Unigene: 229447 Mouse
      • Unigene: 10620 Rat
      see all
    • Alternative names

      • MC4 Receptor antibody
      • MC4-R antibody
      • MC4R antibody
      • MC4R_HUMAN antibody
      • Melanocortin 4 receptor antibody
      • Melanocortin receptor 4 antibody
      • MGC126851 antibody
      • MGC138197 antibody
      see all

    Images

    • Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)
      Western blot - Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)
      All lanes : Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : SH-SY5Y cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate
      Lane 7 : PC12 cell lysate
      Lane 8 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 36 kDa

    • Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)
      Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab150419? Please let us know so that we can cite the reference in this datasheet.

    ab150419 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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