Recombinant Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6534(2)(ABC)] to MC4-R
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MC4-R antibody [EPR6534(2)(ABC)]
See all MC4-R primary antibodies -
Description
Rabbit monoclonal [EPR6534(2)(ABC)] to MC4-R -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide within Human MC4-R aa 1-100 (C terminal). The exact sequence is proprietary.
Database link: P32245 -
Positive control
- 293T; MCF7; JAR; SH-SY5Y; C6; RAW 264.7; PC12 and NIH 3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6534(2)(ABC) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
Target
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Function
Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase. -
Tissue specificity
Brain, placental, and gut tissues. -
Involvement in disease
Defects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4160 Human
- Entrez Gene: 17202 Mouse
- Entrez Gene: 25635 Rat
- Omim: 155541 Human
- SwissProt: P32245 Human
- SwissProt: P56450 Mouse
- SwissProt: P70596 Rat
- Unigene: 532833 Human
see all -
Alternative names
- MC4 Receptor antibody
- MC4-R antibody
- MC4R antibody
see all
Images
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All lanes : Anti-MC4-R antibody [EPR6534(2)(ABC)] (ab150419) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : JAR cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
Datasheets and documents
References (0)
ab150419 has not yet been referenced specifically in any publications.