Overview

  • Product name
    Anti-MCAF1 antibody [2052C2a]
    See all MCAF1 primary antibodies
  • Description
    Mouse monoclonal [2052C2a] to MCAF1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human), amino acids 50-200.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality
    Monoclonal
  • Clone number
    2052C2a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 136 kDa).

Target

  • Relevance
    MCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. It can act both as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. MCAF1 is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Activating transcription factor 7 interacting protein 1 antibody
    • ATF interacting protein antibody
    • ATF IP antibody
    • ATF7IP antibody
    • ATFa associated modulator antibody
    • FLJ10139 antibody
    • FLJ10688 antibody
    • hAM antibody
    • HGNC:20092 antibody
    • MBD1 containing chromatin associated factor antibody
    • MCAF 1 antibody
    • MCAF antibody
    • p621 antibody
    see all

Images

  • Left: Western blot analysis of immunized recombinant protein using ab50956.

    Right: CBB staining of immunized recombinant protein.

References

ab50956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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