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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Kinesin
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Validated using a knockout cell line

Anti-MCAK antibody [2488C3a] (ab50778)

  • Datasheet
  • SDS
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Western blot - Anti-MCAK antibody [2488C3a] (ab50778)
  • Western blot - Anti-MCAK antibody [2488C3a] (ab50778)

Key features and details

  • Mouse monoclonal [2488C3a] to MCAK
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human MCAK protein (ab132006)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-MCAK antibody [2488C3a]
    See all MCAK primary antibodies
  • Description

    Mouse monoclonal [2488C3a] to MCAK
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant human MCAK fragment

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    2488C3a
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human MCAK protein (ab132006)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab50778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.

Target

  • Function

    Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
  • Tissue specificity

    Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
  • Sequence similarities

    Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Developmental stage

    Isoform 2 is expressed in fetal testis.
  • Domain

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
  • Cellular localization

    Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Target information above from: UniProt accession Q99661 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11004 Human
    • Omim: 604538 Human
    • SwissProt: Q99661 Human
    • Unigene: 720061 Human
    • Alternative names

      • 4930402F02Rik antibody
      • CT139 antibody
      • ESTM5 antibody
      • KIF 2C antibody
      • kif2c antibody
      • KIF2C_HUMAN antibody
      • Kinesein Family Member 2C antibody
      • Kinesin family member 2C antibody
      • kinesin like 6 (mitotic centromere associated kinesin) antibody
      • Kinesin like 6 antibody
      • Kinesin like protein 6 antibody
      • Kinesin like protein KIF2C antibody
      • Kinesin-like protein 6 antibody
      • Kinesin-like protein KIF2C antibody
      • KNS L6 antibody
      • KNSL 6 antibody
      • KNSL6 antibody
      • MCAK antibody
      • MGC11883 antibody
      • Mitotic centromere associated kinesin antibody
      • Mitotic centromere-associated kinesin antibody
      • OTTHUMP00000010066 antibody
      • X83316 antibody
      see all

    Images

    • Western blot - Anti-MCAK antibody [2488C3a] (ab50778)
      Western blot - Anti-MCAK antibody [2488C3a] (ab50778)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: MCAK  knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab50778 observed at 90 kDa. Red - loading control, ab176560, observed at 50 kDa.

      ab50778 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. Ab50778 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MCAK antibody [2488C3a] (ab50778)
      Western blot - Anti-MCAK antibody [2488C3a] (ab50778)
      Anti-MCAK antibody [2488C3a] (ab50778) + immunizing peptide

      Predicted band size: 81 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab50778? Please let us know so that we can cite the reference in this datasheet.

    ab50778 has been referenced in 2 publications.

    • D'Angelo L  et al. MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility. Mol Biol Cell 28:1223-1237 (2017). PubMed: 28298485
    • Braun A  et al. Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells. J Cell Biol 206:97-112 (2014). PubMed: 25002679

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