Overview

  • Product name

  • Description

    Rabbit polyclonal to MCAK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MCAK (internal sequence). The exact sequence is proprietary.
    Database link: Q99661

  • Positive control

    • WB: K562 whole cell lysate. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
  • Tissue specificity

    Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
  • Sequence similarities

    Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Developmental stage

    Isoform 2 is expressed in fetal testis.
  • Domain

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
  • Cellular localization

    Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4930402F02Rik antibody
    • CT139 antibody
    • ESTM5 antibody
    • KIF 2C antibody
    • kif2c antibody
    • KIF2C_HUMAN antibody
    • Kinesein Family Member 2C antibody
    • Kinesin family member 2C antibody
    • kinesin like 6 (mitotic centromere associated kinesin) antibody
    • Kinesin like 6 antibody
    • Kinesin like protein 6 antibody
    • Kinesin like protein KIF2C antibody
    • Kinesin-like protein 6 antibody
    • Kinesin-like protein KIF2C antibody
    • KNS L6 antibody
    • KNSL 6 antibody
    • KNSL6 antibody
    • MCAK antibody
    • MGC11883 antibody
    • Mitotic centromere associated kinesin antibody
    • Mitotic centromere-associated kinesin antibody
    • OTTHUMP00000010066 antibody
    • X83316 antibody
    see all

Images

  • Anti-MCAK antibody (ab228016) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 30 µg

    Predicted band size: 81 kDa



    7.5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCAK (green) using ab228016 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counter stain: Hoechst 33342 (blue).

References

ab228016 has not yet been referenced specifically in any publications.

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