Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCAK antibody
See all MCAK primary antibodies
DescriptionRabbit polyclonal to MCAK
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human MCAK (internal sequence). The exact sequence is proprietary.
Database link: Q99661
- WB: K562 whole cell lysate. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 81 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain.
Developmental stageIsoform 2 is expressed in fetal testis.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
- Information by UniProt
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
Anti-MCAK antibody (ab228016) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS-PAGE gel.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCAK (green) using ab228016 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counter stain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228016 has been referenced in 1 publication.
- Stiff T et al. Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation. Cell Rep 31:107681 (2020). PubMed: 32460023