Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCAK antibody
See all MCAK primary antibodies
DescriptionRabbit polyclonal to MCAK
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MCAK aa 675-725 (C terminal). The exact sequence is proprietary.
Database link: Q99661
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
1/2000 - 1/10000.
Use at 2-5 µg/mg of lysate.
1/2000 - 1/10000.
FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain.
Developmental stageIsoform 2 is expressed in fetal testis.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
- Information by UniProt
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
All lanes : Anti-MCAK antibody (ab264149) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab70536, ab264148 and ab264149 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264148 at 1 µg/ml.
Lane 1: ab70536 IP in HeLa whole cell lysate.
Lane 2: ab264148 IP in HeLa whole cell lysate.
Lane 3: ab264149 IP in HeLa whole cell lysate.
Lane 4: Control IgG.
Exposure time: 10 seconds.
MCAK was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264149 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264149 at 1 µg/ml.
Lane 1: ab264149 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264149 has not yet been referenced specifically in any publications.