Anti-MCAK antibody (ab71706)
Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-MCAK antibody
See all MCAK primary antibodies -
Description
Rabbit polyclonal to MCAK -
Host species
Rabbit -
Specificity
Does not react with human MCAK. -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse MCAK. A region between residue 50 and 100 of mouse MCAK. GenBank: NP_608301.2
Database link: Q922S8 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Promotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. -
Tissue specificity
Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. -
Sequence similarities
Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain. -
Developmental stage
Isoform 2 is expressed in fetal testis. -
Domain
The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2. - Information by UniProt
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Database links
- Entrez Gene: 73804 Mouse
- Entrez Gene: 171529 Rat
- SwissProt: Q922S8 Mouse
- SwissProt: Q62909 Rat
- Unigene: 247651 Mouse
- Unigene: 219966 Rat
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Alternative names
- 4930402F02Rik antibody
- CT139 antibody
- ESTM5 antibody
see all
Images
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All lanes : Anti-MCAK antibody (ab71706) at 0.1 µg/ml
Lane 1 : NIH3T3 whole cell lysate at 50 µg/ml
Lane 2 : NIH3T3 whole cell lysate at 15 µg/ml
Lane 3 : NIH3T3 whole cell lysate at 5 µg/ml
Predicted band size: 81 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse hybridoma tumor tissue labelling MCAK with ab71706 at 1/1000 (1µg/ml). Detection: DAB.
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MCAK antibody (ab71706) recognizing MCAK protein in NIH3T3 whole cell lysate (1 mg, 20% of IP loaded).
Lane 1: MCAK antibody (ab71706) used for IP at 3 µg/mg lysate and for WB at 1.0 µg/ml.
Lane 2: Non-Abcam MCAK antibody, specific for a downstream epitope of MCAK.
Lane 3: Control IgG.
Detection by chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab71706 has been referenced in 1 publication.
- Huang CH et al. Identification of aberrantly methylated differentially expressed genes and pro-tumorigenic role of KIF2C in melanoma. Front Genet 13:817656 (2022). PubMed: 35991567