Key features and details
- Rabbit polyclonal to MCAK
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-MCAK antibody
See all MCAK primary antibodies
DescriptionRabbit polyclonal to MCAK
SpecificityDoes not react with human MCAK.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Synthetic peptide corresponding to Mouse MCAK. A region between residue 50 and 100 of mouse MCAK.
Database link: NP_608301.2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionPromotes ATP-dependent removal of tubulin dimers from microtubules. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Tissue specificityExpressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.
Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
Contains 1 kinesin-motor domain.
Developmental stageIsoform 2 is expressed in fetal testis.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by STK12, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Centromeric localization requires the presence of BUB1 and SGOL2.
- Information by UniProt
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All lanes : Anti-MCAK antibody (ab71706) at 0.1 µg/ml
Lane 1 : NIH3T3 whole cell lysate at 50 µg/ml
Lane 2 : NIH3T3 whole cell lysate at 15 µg/ml
Lane 3 : NIH3T3 whole cell lysate at 5 µg/ml
Predicted band size: 81 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse hybridoma tumor tissue labelling MCAK with ab71706 at 1/1000 (1µg/ml). Detection: DAB.
MCAK antibody (ab71706) recognizing MCAK protein in NIH3T3 whole cell lysate (1 mg, 20% of IP loaded).
Lane 1: MCAK antibody (ab71706) used for IP at 3 µg/mg lysate and for WB at 1.0 µg/ml.
Lane 2: Non-Abcam MCAK antibody, specific for a downstream epitope of MCAK.
Lane 3: Control IgG.
Detection by chemiluminescence with exposure time of 30 seconds.
ab71706 has not yet been referenced specifically in any publications.