Overview

  • Product name

    Anti-MCEE antibody [OTI1B5]
  • Description

    Mouse monoclonal [OTI1B5] to MCEE
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human MCEE. Produced in E.coli (NP_115990).
    Database link: Q96PE7

  • Positive control

    • WB: pCMV6-ENTRY MCEE cDNA transfected HEK-293T cell lysate. IHC-P: Human colon adenocarcinoma, pancreas carcinoma, endometrium adenocarcinoma, prostate, prostate adenocarcinoma and bladder tissue.
  • General notes

    Clone OTI1B5 (formerly 1B5).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI1B5
  • Isotype

    IgG2b

Applications

Our Abpromise guarantee covers the use of ab236397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 19 kDa.
IHC-P 1/150.

Heat-induced epitope retrieval by Tris-EDTA, pH 8.0.

Target

  • Involvement in disease

    Defects in MCEE are a cause of methylmalonyl-CoA epimerase deficiency (MCEE deficiency) [MIM:251120]; also known as methylmalonyl-CoA racemase deficiency or methylmalonic aciduria type 3. MCEE deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma.
  • Sequence similarities

    Belongs to the glyoxalase I family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • DL methylmalonyl CoA racemase antibody
    • DL-methylmalonyl-CoA racemase antibody
    • EC 5.1.99.1 antibody
    • GLOD2 antibody
    • Glyoxalase domain containing 2 antibody
    • MCEE antibody
    • MCEE_HUMAN antibody
    • Methylmalonyl CoA epimerase antibody
    • Methylmalonyl-CoA epimerase antibody
    • methylmalonyl-CoA epimerase, mitochondrial antibody
    • mitochondrial antibody
    • OTTHUMP00000160122 antibody
    see all

Images

  • All lanes : Anti-MCEE antibody [OTI1B5] (ab236397) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY MCEE cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 19 kDa

  • Paraffin-embedded human bladder tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

  • Paraffin-embedded human prostate carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

  • Paraffin-embedded human prostate tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

  • Paraffin-embedded human endometrium adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

  • Paraffin-embedded human pancreas carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

  • Paraffin-embedded human colon adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.

References

ab236397 has not yet been referenced specifically in any publications.

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