Product nameMCF7 nuclear extract lysate
See all MCF7 lysates
Extracts have been quality control tested by Western blot.
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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MCF7 nuclear extract lysate was prepared from cell cultures of the human MCF7 breast adenocarcinoma cancer cell line. The MCF7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer
BackgroundMCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14860 has not yet been referenced specifically in any publications.