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Kits/ Lysates/ Other Lysates Nuclear Lysates Human Other
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MCF7 nuclear extract lysate (H2O2 treated) (ab14862)

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Functional Studies - MCF7 nuclear extract lysate (H<sub>2</sub>O<sub>2</sub> treated) (ab14862)

    Overview

    • Product name

      MCF7 nuclear extract lysate (H2O2 treated)
      See all MCF7 lysates
    • Description

      MCF7 nuclear extract lysate
    • General notes

      The MCF-7 nuclear extracts have been collected in Lysis Buffer after 3 hrs incubation with or without 0.2 mM hydrogen peroxide. The protein content has been determined by a Bradford-based assay.

    Properties

    • Mycoplasma free

      Yes
    • Form

      Liquid
    • Storage instructions

      Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.50
      Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
    • Concentration information loading...
    • Research areas

      • Kits/ Lysates/ Other
      • Lysates
      • Nuclear Lysates
      • Human
      • Other
    • Background

      MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.

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    • Functional Studies - MCF7 nuclear extract lysate (H<sub>2</sub>O<sub>2</sub> treated) (ab14862)
      Functional Studies - MCF7 nuclear extract lysate (H2O2 treated) (ab14862)

       Each lot has been tested for p53 activation by using TransAM™ p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated cells. Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 µg/well is above 4. This ratio may vary depending on the basal level of p53 activation in a given cell type.

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

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  • References (0)

    Publishing research using ab14862? Please let us know so that we can cite the reference in this datasheet.

    ab14862 has not yet been referenced specifically in any publications.

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