Overview

  • Product name
  • Description
    Rabbit polyclonal to MCH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MCH aa 101-130 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P20382

  • Positive control
    • K562 cells and cell line lysates; Mouse brain tissue lysates; Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174886 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.

Target

  • Relevance
    Melanin Concentrating Hormone (MCH) is the ligand of the somatostatin-like receptor 1 (SLC1 or MCH Receptor). This hormone induces melanosomal aggregation within melanophores and has orexigenic properties and is antagonistic to melanotropin. MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Melanin concentrating hormone antibody
    • PMCH antibody
    • Pro MCH antibody
    • Pro melanin concentrating hormone antibody

Images

  • Anti-MCH antibody (ab174886) at 1/100 dilution + K562 cell line lysates at 15 µg

    Predicted band size: 19 kDa

  • Anti-MCH antibody (ab174886) at 1/100 dilution + Mouse brain tissue lysates at 15 µg

    Predicted band size: 19 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling MCH with ab174886 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells labeling MCH with ab174886 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References

ab174886 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174886.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up