Anti-mCherry antibody [1C51] (ab125096)
Key features and details
- Mouse monoclonal [1C51] to mCherry
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-mCherry antibody [1C51]
See all mCherry primary antibodies -
Description
Mouse monoclonal [1C51] to mCherry -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to mCherry.
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Positive control
- IHC-P: mCherry-transfected monkey brain tissue, Drosophila melanogaster eye imaginal discs and smooth muscle cells in rat bladder tissue. WB: HEK-293 cells transfected with mCherry-HA construct and mCherry recombinant protein. ICC/IF: mCherry-transfected HEK-293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1C51 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/2000. Detects a band of approximately 28 kDa.
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ICC/IF | (3) |
1/500.
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IHC-P | (4) |
1/2000.
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Notes |
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WB
1/2000. Detects a band of approximately 28 kDa. |
ICC/IF
1/500. |
IHC-P
1/2000. |
Target
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Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
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Chromogenic immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, dilution 1/2000, detected in DAB (brown) following the ImmPress method. Hematoxylin (blue) was used as the counterstain. ab125096 specifically detected the soma and axons of mCherry positive neurons in the cortex, as expected for this model.
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4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.
Analysis of rin hypomorphic alleles and the genomic rescue transgene GrinCherry. (A–C'') Negatively marked 72 h old rin2 (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the GrinCherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 µm.
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All lanes : Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HEK-293 cells transfected with mCherry-HA construct
Lane 4 : mCherry recombinant protein
Lane 5 : GFP recombinant protein
Lane 6 : HEK-293 transfected with GFP construct.Major band at about 30kDa corresponds to mCherry protein.
MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.
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mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour.
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ab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (175)
ab125096 has been referenced in 175 publications.
- Xia B et al. Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2. Cell Discov 9:2 (2023). PubMed: 36609376
- Quintanilla CG et al. Nir1 constitutively localizes at ER-PM junctions and promotes Nir2 recruitment for PIP2 homeostasis. Mol Biol Cell 33:br2 (2022). PubMed: 35020418
- Liu Y et al. Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene. Gene Ther 29:368-378 (2022). PubMed: 35383288
- Shkarina K et al. Optogenetic activators of apoptosis, necroptosis, and pyroptosis. J Cell Biol 221:N/A (2022). PubMed: 35420640
- Ferrandiz N et al. Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes. J Cell Biol 221:N/A (2022). PubMed: 35486148