Overview

  • Product name

    Anti-mCherry antibody [1C51]
    See all mCherry primary antibodies
  • Description

    Mouse monoclonal [1C51] to mCherry
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein corresponding to mCherry.

  • Positive control

    • ICC/IF: pFin-EF1-mCherry transfected HEK-293T cells. Rat bladder. IHC-Fr: Human pancreatic islet tissue; Rat brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.03% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1C51
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 28 kDa.
ICC/IF 1/500.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Images

  • 4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.

    Analysis of rin hypomorphic alleles and the genomic rescue transgene GrinCherry. (A–C'') Negatively marked 72 h old rin2 (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the GrinCherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 µm.

  • All lanes : Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution

    Lane 1 : Protein standard
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : HEK-293 cells transfected with mCherry-HA construct
    Lane 4 : mCherry recombinant protein
    Lane 5 : GFP recombinant protein
    Lane 6 : HEK-293 transfected with GFP construct.


    Major band at about 30kDa corresponds to mCherry protein.

    MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.

  • Immuohistochemical analysis of frozen rat brain sections (40 µm sections). Sections were washed in PBS and subsequently blocked and permeabilized in PBS containing 10% normal goat serum and 0.05% Tween-20 for 1 h. After washing in PBS, sections were incubated overnight with ab125096 (Red) at 1/500 dilution in PBS containing 0.05% Tween-20. After washing in PBS, sections were incubated with Alexa-594 labelled goat anti-mouse IgG secondary for 1 h.

    The upper row depicts sindle section images of (A) hM3D(Gq)-mCherry expression (B) Tyrosine Hyrdoxylase expression (C) Merged image of A and B. hM3D(Gq)-mCherry expression is restricted to the ventral tegmental area (VTA), as no mCherry expression could be observed in the substantia nigra pars compacta (SNpc) or surrounding areas.

    The lower row depicts single section sonfocal images of (D) hM3D(Gq)-mCherry expression (E) TH expression and (F) Merged of D and E. Two populations of hM3D(Gq)-mCherry expressing VTA to nucleus accumbens (Acb) projection neurons can be distinguished, one population that does not express TH (vertical white arrows) and one population that does express TH (horizontal yellow arrows).

  • mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour. 

  • ab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.

    Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.

    See Abreview

  • ab125096 staining mCherry in human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

    Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with BSA + milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Vilà-González M  et al. Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling. Stem Cells 37:226-239 (2019). Read more (PubMed: 30372556) »
  • Scrivo A  et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun 10:780 (2019). Read more (PubMed: 30770803) »
See all 77 Publications for this product

Customer reviews and Q&As

1-10 of 42 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
Expressing mCherry+fragment of 53BP1
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 08 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 06 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 18 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Kathryn Baker

Verified customer

Submitted Mar 15 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (xenograft of mCherry labelled human (HUVEC) cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Permeabilization
No
Specification
xenograft of mCherry labelled human (HUVEC) cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 12 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole Head Lysate)
Specification
Whole Head Lysate
Blocking step
Odyssey as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 23 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde

Dr. Karine Thibault

Verified customer

Submitted Nov 04 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (BT474 Cells)
Specification
BT474 Cells
Permeabilization
Yes - 1% NP40
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 06 2014

Question
Answer


I can confirm that we have tested this antibody with DsRed and it works well.

Read More
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA+Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Tissue sections (Pancreatic islet)
Specification
Pancreatic islet
Permeabilization
Yes - Triton X-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 11 2013

1-10 of 42 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up