Product nameAnti-mCherry antibody [1C51]
See all mCherry primary antibodies
DescriptionMouse monoclonal [1C51] to mCherry
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Species independent
Recombinant full length protein corresponding to mCherry.
- ICC/IF: pFin-EF1-mCherry transfected HEK-293T cells. Rat bladder. IHC-Fr: Human pancreatic islet tissue; Rat brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab125096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 28 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
RelevancemCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
4% paraformaldehyde fixed (at RT for 20 min and blocked with 2% NDS in 0.3% PBT) Drosophila melanogaster eye imaginal discs stained for mCherry (Red) using ab125096.
Analysis of rin hypomorphic alleles and the genomic rescue transgene GrinCherry. (A–C'') Negatively marked 72 h old rin2 (A, A''), (C, C'') mutant clones (induced with the FLP/FRT system) in eye imaginal discs of third instar larvae. Rin-Cherry levels expressed from the GrinCherry are autonomously increased in the rin mutant clones (A' and C'). The scale bar represents 50 µm.
All lanes : Anti-mCherry antibody [1C51] (ab125096) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : HEK-293 cells transfected with mCherry-HA construct
Lane 4 : mCherry recombinant protein
Lane 5 : GFP recombinant protein
Lane 6 : HEK-293 transfected with GFP construct.
Major band at about 30kDa corresponds to mCherry protein.
MCA-1C51 antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals band at 60kDa seen only in cell lysates.
Immuohistochemical analysis of frozen rat brain sections (40 µm sections). Sections were washed in PBS and subsequently blocked and permeabilized in PBS containing 10% normal goat serum and 0.05% Tween-20 for 1 h. After washing in PBS, sections were incubated overnight with ab125096 (Red) at 1/500 dilution in PBS containing 0.05% Tween-20. After washing in PBS, sections were incubated with Alexa-594 labelled goat anti-mouse IgG secondary for 1 h.
The upper row depicts sindle section images of (A) hM3D(Gq)-mCherry expression (B) Tyrosine Hyrdoxylase expression (C) Merged image of A and B. hM3D(Gq)-mCherry expression is restricted to the ventral tegmental area (VTA), as no mCherry expression could be observed in the substantia nigra pars compacta (SNpc) or surrounding areas.
The lower row depicts single section sonfocal images of (D) hM3D(Gq)-mCherry expression (E) TH expression and (F) Merged of D and E. Two populations of hM3D(Gq)-mCherry expressing VTA to nucleus accumbens (Acb) projection neurons can be distinguished, one population that does not express TH (vertical white arrows) and one population that does express TH (horizontal yellow arrows).
mCherry-transfected HEK-293 (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling mCherry using ab125096 followed with a green secondary. DAPI was used to stain nuclei in blue. Cells are visualized using a confocal microscope. Transfected cells appear in yellow/orange colour.
ab125096 staining mCherry in smooth muscle cells in rat bladder by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
Tissue was fixed with 10% buffered formalin and blocked with 5000 ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
ab125096 staining mCherry in human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with BSA + milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
This product has been referenced in:
- Vilà-González M et al. Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling. Stem Cells 37:226-239 (2019). Read more (PubMed: 30372556) »
- Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun 10:780 (2019). Read more (PubMed: 30770803) »