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    mcherry-antibody-ab167453.pdf

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Anti-mCherry antibody (ab167453)

  • Datasheet
  • SDS
Reviews (17)Q&A (4)References (204)

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Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)
  • Western blot - Anti-mCherry antibody (ab167453)

Key features and details

  • Rabbit polyclonal to mCherry
  • Suitable for: WB, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG

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Primary
Agarose Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1240)
Protein
Recombinant mCherry protein (His tag) (ab199750)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-mCherry antibody
    See all mCherry primary antibodies
  • Description

    Rabbit polyclonal to mCherry
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant full length protein corresponding to mCherry.

  • General notes

      

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Kits/ Lysates/ Other
    • Fluorescent ligands

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant mCherry protein (His tag) (ab199750)
  • Related Products

    • mCherry ELISA Kit (ab221829)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000.
ICC/IF (4)
1/500.
Notes
WB
1/1000.
ICC/IF
1/500.

Target

  • Relevance

    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)
    Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)
    Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.
  • Western blot - Anti-mCherry antibody (ab167453)
    Western blot - Anti-mCherry antibody (ab167453)
    All lanes : Anti-mCherry antibody (ab167453) at 1/1000 dilution

    Lane 1 :


    HEK293
    cells transfected with pFin-
    EF1-mCherry vector
    Lane 2 :


    Non-transfected HEK293
    cells



Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (204)

Publishing research using ab167453? Please let us know so that we can cite the reference in this datasheet.

ab167453 has been referenced in 204 publications.

  • Tambalo M  et al. Enhancer activation by FGF signalling during otic induction. Dev Biol 457:69-82 (2020). PubMed: 31539539
  • Bodrug T  et al. The kinesin-5 tail domain directly modulates the mechanochemical cycle of the motor domain for anti-parallel microtubule sliding. Elife 9:N/A (2020). PubMed: 31958056
  • Corbet C  et al. TGFß2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells. Nat Commun 11:454 (2020). PubMed: 31974393
  • Nowacka A  et al. PSD-95 Serine 73 phosphorylation is not required for induction of NMDA-LTD. Sci Rep 10:2054 (2020). PubMed: 32029829
  • Le Guennec M  et al. A helical inner scaffold provides a structural basis for centriole cohesion. Sci Adv 6:eaaz4137 (2020). PubMed: 32110738
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 21 Abreviews or Q&A

Western blot abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese hamster ovary cells)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
25 µg
Specification
Chinese hamster ovary cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Chansik Yoon

Verified customer

Submitted Feb 01 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mCherry antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse knee)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
mouse knee
Blocking step
Bloxall and Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
zinc buffered formalin
Read More

Abcam user community

Verified customer

Submitted Aug 28 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Permeabilization
Yes - Saponin 0.1%
Specification
HeLa cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Mrs. Federica Ferrentino

Verified customer

Submitted Feb 28 2020

Immunohistochemistry (Frozen sections) abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - 0.3% Triton X-100
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 25 2019

Western blot abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10% NuPAGE bis Tris)
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Feb 14 2019

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 12 2018

Immunohistochemistry free floating abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Read More

Dr. Alexandre Magno

Verified customer

Submitted Aug 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - 3% H2O2/Methanol for 10min, RT
Specification
Lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 15 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.25% triton
Specification
U2OS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 24 2017

Immunohistochemistry free floating abreview for Anti-mCherry antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Read More

Abcam user community

Verified customer

Submitted Jan 24 2017

1-10 of 21 Abreviews or Q&A

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