Anti-mCherry antibody (ab167453)
Key features and details
- Rabbit polyclonal to mCherry
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Overview
-
Product name
Anti-mCherry antibody
See all mCherry primary antibodies -
Description
Rabbit polyclonal to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to mCherry.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000.
|
| ICC/IF | (4) |
1/500.
|
| Notes |
|---|
|
WB
1/1000. |
|
ICC/IF
1/500. |
Target
-
Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.
-
Western blot - Anti-mCherry antibody (ab167453)All lanes : Anti-mCherry antibody (ab167453) at 1/1000 dilution
Lane 1 :
HEK293
cells transfected with pFin-
EF1-mCherry vector
Lane 2 :
Non-transfected HEK293
cells
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (204)
ab167453 has been referenced in 204 publications.
- Tambalo M et al. Enhancer activation by FGF signalling during otic induction. Dev Biol 457:69-82 (2020). PubMed: 31539539
- Bodrug T et al. The kinesin-5 tail domain directly modulates the mechanochemical cycle of the motor domain for anti-parallel microtubule sliding. Elife 9:N/A (2020). PubMed: 31958056
- Corbet C et al. TGFß2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells. Nat Commun 11:454 (2020). PubMed: 31974393
- Nowacka A et al. PSD-95 Serine 73 phosphorylation is not required for induction of NMDA-LTD. Sci Rep 10:2054 (2020). PubMed: 32029829
- Le Guennec M et al. A helical inner scaffold provides a structural basis for centriole cohesion. Sci Adv 6:eaaz4137 (2020). PubMed: 32110738