Overview

  • Product name
  • Description
    Rabbit polyclonal to mCherry
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Immunogen

    Recombinant full length protein corresponding to mCherry.

  • Positive control
    • HEK293 cells transfected with pFin- EF1-mCherry vector.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC/IF 1/500.
IHC-P Use a concentration of 3.33 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Images

  • Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.
  • All lanes : Anti-mCherry antibody (ab167453) at 1/1000 dilution

    Lane 1 :


    HEK293
    cells transfected with pFin-
    EF1-mCherry vector
    Lane 2 :


    Non-transfected HEK293
    cells



  • Immunohistochemical analysis of paraffin-embedded mouse lung metastases sections labelling mCherry with ab167453 at a concentration of 3.33 µg/mL. The secondary antibody used was HRP conjugated goat anti rabbit at a concentration of 2 µg/mL. Antigen retrieval was heat mediated using Menapath access super pH 9.5.

    See Abreview

References

This product has been referenced in:
  • Webber HC  et al. The Role of Wnt/ß-Catenin Signaling and K-Cadherin in the Regulation of Intraocular Pressure. Invest Ophthalmol Vis Sci 59:1454-1466 (2018). Read more (PubMed: 29625468) »
  • Qamar S  et al. FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-p Interactions. Cell 173:720-734.e15 (2018). Read more (PubMed: 29677515) »

See all 47 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - 3% H2O2/Methanol for 10min, RT
Specification
Lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 15 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.25% triton
Specification
U2OS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 24 2017

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

Submitted Jan 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H7.S6 Human ES cells with mCherry gene reporter)
Permeabilization
Yes - 0.1% Triton X
Specification
H7.S6 Human ES cells with mCherry gene reporter
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung metastases)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.5 Menapath access super
Permeabilization
No
Specification
Lung metastases
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Mr. Bruce Fanshawe

Verified customer

Submitted Jun 03 2016


We have not tested this antibody for reactivity with tdTomato but it is likely to cross react. One customer has reported successful use with tDTomato.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mCherry positive tumour cells in lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH: 6
Permeabilization
No
Specification
mCherry positive tumour cells in lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
10% Buffered Formalin
Username

Alex Spurling

Verified customer

Submitted May 12 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Humen leukemia cells with mCherry in murine bone)
Permeabilization
Yes - 0.1% Tween
Specification
Humen leukemia cells with mCherry in murine bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formalin
Username

Abcam user community

Verified customer

Submitted Apr 20 2015

Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing (10 percent gel)
Sample
Human Purified protein (mCherry against various amounts of nanograms of pu)
Specification
mCherry against various amounts of nanograms of pu
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2015

We do not have data for cross-reactivity of the mCherry antibodies we offer, ab167453, ab125096, or ab183628, with GFP or CFP. However, reactivity is expected to be low or absent, given the low homology between mCherry and GFP/CFP.

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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