Product nameAnti-mCherry antibody
See all mCherry primary antibodies
DescriptionRabbit polyclonal to mCherry
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Species independent
Recombinant full length protein corresponding to mCherry.
- HEK293 cells transfected with pFin- EF1-mCherry vector.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.065% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab167453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3.33 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevancemCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain.
All lanes : Anti-mCherry antibody (ab167453) at 1/1000 dilution
Lane 1 :
cells transfected with pFin-
Lane 2 :
Immunohistochemical analysis of paraffin-embedded mouse lung metastases sections labelling mCherry with ab167453 at a concentration of 3.33 µg/mL. The secondary antibody used was HRP conjugated goat anti rabbit at a concentration of 2 µg/mL. Antigen retrieval was heat mediated using Menapath access super pH 9.5.
This product has been referenced in:
- López-Ferreras L et al. GLP-1 modulates the supramammillary nucleus-lateral hypothalamic neurocircuit to control ingestive and motivated behavior in a sex divergent manner. Mol Metab 20:178-193 (2019). Read more (PubMed: 30528281) »
- Otis JP et al. Dietary cholesterol and apolipoprotein A-I are trafficked in endosomes and lysosomes in the live zebrafish intestine. Am J Physiol Gastrointest Liver Physiol 316:G350-G365 (2019). Read more (PubMed: 30629468) »