Anti-mCherry antibody (ab167453)
Rabbit polyclonal mCherry antibody. Validated in WB, IHC, ICC/IF. Cited in 71 publication(s). Independently reviewed in 12 review(s).
- Datasheet
- References (144)
- Protocols
Overview
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Product name
Anti-mCherry antibody
See all mCherry primary antibodies -
Description
Rabbit polyclonal to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to mCherry.
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Positive control
- HEK293 cells transfected with pFin- EF1-mCherry vector.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab167453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. | |
| ICC/IF | 1/500. | |
| IHC-P | Use a concentration of 3.33 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
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Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody (ab167453)Immunofluorescent analysis of HEK293 cells transfected with pFin- EF1-mCherry vector labeling mCherry with ab167453 at 1/500 (red). Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Blue DNA stain. -
Western blot - Anti-mCherry antibody (ab167453)All lanes : Anti-mCherry antibody (ab167453) at 1/1000 dilution
Lane 1 :
HEK293
cells transfected with pFin-
EF1-mCherry vector
Lane 2 :
Non-transfected HEK293
cells
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mCherry antibody (ab167453)This image is courtesy of an abreview submitted by Bruce FanshaweImmunohistochemical analysis of paraffin-embedded mouse lung metastases sections labelling mCherry with ab167453 at a concentration of 3.33 µg/mL. The secondary antibody used was HRP conjugated goat anti rabbit at a concentration of 2 µg/mL. Antigen retrieval was heat mediated using Menapath access super pH 9.5.
Protocols
References
This product has been referenced in:
- López-Ferreras L et al. GLP-1 modulates the supramammillary nucleus-lateral hypothalamic neurocircuit to control ingestive and motivated behavior in a sex divergent manner. Mol Metab 20:178-193 (2019). Read more (PubMed: 30528281) »
- Otis JP et al. Dietary cholesterol and apolipoprotein A-I are trafficked in endosomes and lysosomes in the live zebrafish intestine. Am J Physiol Gastrointest Liver Physiol 316:G350-G365 (2019). Read more (PubMed: 30629468) »
