Anti-mCherry antibody (ab205402)
Key features and details
- Chicken polyclonal to mCherry
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgY
Overview
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Product name
Anti-mCherry antibody
See all mCherry primary antibodies -
Description
Chicken polyclonal to mCherry -
Host species
Chicken -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein (His-tag) corresponding to mCherry. The immunogen sequence is from a following reference. Shaner NC et al. Nature Biotechnology 22:1567-1572 (2004).
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Positive control
- Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector; HEK293 cells transfected with pFin-EF1-mCherry vector.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.0325% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Purification notes
Concentrated IgY preparation. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 30 kDa.
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ICC/IF | (1) |
1/1000.
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Notes |
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WB
1/2000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/1000. |
Target
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Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
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Immunofluorescent analysis of HEK293 cells transfected with pFin-EF1-mCherry vector labeling mCherry using ab205402 at 1/1000 dilution (green). Red: mCherry; Blue: blue DNA stain. Note: Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal.
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Anti-mCherry antibody (ab205402) at 1/2000 dilution + lysate of HEK293 cells transfected with pFin-EF1-mCherry vector
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Immunocytochemistry/ Immunofluorescence analysis of Human ES cells with mCherry gene reporter labeling mCherry with ab205402 at 1/1000 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X. Blocking was carried out with 10%BSA for 1 hour at 4°C, followed by incubation with ab205402 at 1/1000 for 12 hours at 4°C. A polyclonal goat anti-chicken Alexa Fluor® 488 was used as the secondary antibody at 1/100 diluton.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (54)
ab205402 has been referenced in 54 publications.
- Kauer SD et al. Inositol Polyphosphate-5-Phosphatase K (Inpp5k) Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma. J Neurosci 42:2190-2204 (2022). PubMed: 35135857
- Sun X et al. Deep Single-Cell-Type Proteome Profiling of Mouse Brain by Nonsurgical AAV-Mediated Proximity Labeling. Anal Chem 94:5325-5334 (2022). PubMed: 35315655
- Asencor AI et al. Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S. eNeuro 9:N/A (2022). PubMed: 35610024
- Frankowski JC et al. Brain-wide reconstruction of inhibitory circuits after traumatic brain injury. Nat Commun 13:3417 (2022). PubMed: 35701434
- Takahashi A et al. Lateral habenula glutamatergic neurons projecting to the dorsal raphe nucleus promote aggressive arousal in mice. Nat Commun 13:4039 (2022). PubMed: 35864121