Overview

  • Product name

  • Description

    Chicken polyclonal to mCherry
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to mCherry. The immunogen sequence is from a following reference. Shaner NC et al. Nature Biotechnology 22:1567-1572 (2004).

  • Positive control

    • Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector; HEK293 cells transfected with pFin-EF1-mCherry vector.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.0325% Sodium azide
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    Concentrated IgY preparation.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 30 kDa.
ICC/IF 1/1000.

Target

  • Relevance

    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Images

  • Immunofluorescent analysis of HEK293 cells transfected with pFin-EF1-mCherry vector labeling mCherry using ab205402 at 1/1000 dilution (green). Red: mCherry; Blue: blue DNA stain. Note: Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal.

  • Anti-mCherry antibody (ab205402) at 1/2000 dilution + lysate of HEK293 cells transfected with pFin-EF1-mCherry vector

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence analysis of Human ES cells with mCherry gene reporter labeling mCherry with ab205402 at 1/1000 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X. Blocking was carried out with 10%BSA for 1 hour at 4°C, followed by incubation with ab205402 at 1/1000 for 12 hours at 4°C. A polyclonal goat anti-chicken Alexa Fluor® 488 was used as the secondary antibody at 1/100 diluton.

    See Abreview

References

This product has been referenced in:

  • Moreno E  et al. Competition for Space Induces Cell Elimination through Compaction-Driven ERK Downregulation. Curr Biol 29:23-34.e8 (2019). Read more (PubMed: 30554899) »
  • Dickie AC  et al. Morphological and functional properties distinguish the substance P and gastrin-releasing peptide subsets of excitatory interneuron in the spinal cord dorsal horn. Pain 160:442-462 (2019). Read more (PubMed: 30247267) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 28 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Trigeminal ganglion)
Permeabilization
Yes - 1% triton, 30 minutes
Specification
Trigeminal ganglion
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Guennadi Dvoriantchikov

Verified customer

Submitted Feb 08 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H7.S6 Human ES cells with mCherry gene reporter)
Permeabilization
Yes - 0.1% Triton-X
Specification
H7.S6 Human ES cells with mCherry gene reporter
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 30 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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