Key features and details
- Chicken polyclonal to mCherry
- Suitable for: WB, ICC/IF
- Isotype: IgY
Product nameAnti-mCherry antibody
See all mCherry primary antibodies
DescriptionChicken polyclonal to mCherry
Tested applicationsSuitable for: WB, ICC/IFmore details
Recombinant full length protein (His-tag) corresponding to mCherry. The immunogen sequence is from a following reference. Shaner NC et al. Nature Biotechnology 22:1567-1572 (2004).
- Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector; HEK293 cells transfected with pFin-EF1-mCherry vector.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.0325% Sodium azide
Concentration information loading...
Purification notesConcentrated IgY preparation.
Our Abpromise guarantee covers the use of ab205402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 30 kDa.|
RelevancemCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Immunofluorescent analysis of HEK293 cells transfected with pFin-EF1-mCherry vector labeling mCherry using ab205402 at 1/1000 dilution (green). Red: mCherry; Blue: blue DNA stain. Note: Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal.
Anti-mCherry antibody (ab205402) at 1/2000 dilution + lysate of HEK293 cells transfected with pFin-EF1-mCherry vector
Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunocytochemistry/ Immunofluorescence analysis of Human ES cells with mCherry gene reporter labeling mCherry with ab205402 at 1/1000 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X. Blocking was carried out with 10%BSA for 1 hour at 4°C, followed by incubation with ab205402 at 1/1000 for 12 hours at 4°C. A polyclonal goat anti-chicken Alexa Fluor® 488 was used as the secondary antibody at 1/100 diluton.
ab205402 has been referenced in 15 publications.
- Sagoshi S et al. Detection and Characterization of Estrogen Receptor Beta Expression in the Brain with Newly Developed Transgenic Mice. Neuroscience 438:182-197 (2020). PubMed: 32387645
- Han Y et al. Excitatory VTA to DH projections provide a valence signal to memory circuits. Nat Commun 11:1466 (2020). PubMed: 32193428
- Tai-Schmiedel J et al. Human cytomegalovirus long noncoding RNA4.9 regulates viral DNA replication. PLoS Pathog 16:e1008390 (2020). PubMed: 32294138
- Yang X et al. SMART-Q: An Integrative Pipeline Quantifying Cell Type-Specific RNA Transcription. PLoS One 15:e0228760 (2020). PubMed: 32348304
- Azevedo EP et al. A limbic circuit selectively links active escape to food suppression. Elife 9:N/A (2020). PubMed: 32894221