Recombinant Anti-mCherry antibody [EPR20579] (ab213511)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
Anti-mCherry antibody [EPR20579]
See all mCherry primary antibodies -
Description
Rabbit monoclonal [EPR20579] to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, Flow Cyt, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein within mCherry aa 1 to the C-terminus. The exact sequence is proprietary. Anaplasma marginale.
Database link: X5DSL3 -
Positive control
- WB: 293T cells transfected with a His-tagged mCherry whole cell lysate. Flow Cyt: 293T cells transfected with a His-tagged mCherry. IP: 293T cells transfected with a His-tagged mCherry whole cell lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20579 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab213511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IP | 1/20. | |
| Flow Cyt | 1/400. | |
| WB | 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa). |
Target
-
Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
-
![Flow Cytometry - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280623-anti-mcherry-antibody-epr20579-flow-cytometry.jpg)
Flow Cytometry - Anti-mCherry antibody [EPR20579] (ab213511)Flow cytometric analysis of 4% paraformaldehyde-fixed 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry cell line labeling mCherry with ab213511 at 1/400 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
mCherry positive population gives positive results as shown in right panel (Q2).
-
![Western blot - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280627-anti-mcherry-antibody-epr20579-western-blot.jpg)
Western blot - Anti-mCherry antibody [EPR20579] (ab213511)All lanes : Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty expression vector whole cell lysate
Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280626-anti-mcherry-antibody-epr20579-western-blot.jpg)
Western blot - Anti-mCherry antibody [EPR20579] (ab213511)Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution + Green fluorescent protein (GFP) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Green fluorescent protein (GFP) contains aa1-238 with His-tag.
-
![Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280620-anti-mcherry-antibody-epr20579-immunoprecipitation.jpg)
Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: 293T transfected with a His-tagged mCherry whole cell lysate 10 µg (Input).
Lane 2: ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Williams JL et al. Conditional Synaptic Vesicle Markers for Drosophila. G3 (Bethesda) 9:737-748 (2019). Read more (PubMed: 30635441) »
- Flores BN et al. An Intramolecular Salt Bridge Linking TDP43 RNA Binding, Protein Stability, and TDP43-Dependent Neurodegeneration. Cell Rep 27:1133-1150.e8 (2019). Read more (PubMed: 31018129) »
