Recombinant Anti-mCherry antibody [EPR20579] (ab213511)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20579] to mCherry
- Suitable for: ELISA, IP, WB
- Reacts with: Species independent
Overview
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Product name
Anti-mCherry antibody [EPR20579]
See all mCherry primary antibodies -
Description
Rabbit monoclonal [EPR20579] to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant fragment within mCherry aa 1 to the C-terminus. The exact sequence is proprietary. Anaplasma marginale.
Database link: X5DSL3 -
Positive control
- WB: 293T cells transfected with a His-tagged mCherry whole cell lysate. IP: 293T cells transfected with a His-tagged mCherry whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20579 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab213511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IP | 1/20. | |
| WB | 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa). |
Target
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Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
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![Western blot - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280627-anti-mcherry-antibody-epr20579-western-blot.jpg)
Western blot - Anti-mCherry antibody [EPR20579] (ab213511)All lanes : Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty expression vector whole cell lysate
Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280626-anti-mcherry-antibody-epr20579-western-blot.jpg)
Western blot - Anti-mCherry antibody [EPR20579] (ab213511)Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution + Green fluorescent protein (GFP) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Green fluorescent protein (GFP) contains aa1-238 with His-tag.
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![Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280620-anti-mcherry-antibody-epr20579-immunoprecipitation.jpg)
Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: 293T transfected with a His-tagged mCherry whole cell lysate 10 µg (Input).
Lane 2: ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-5-benefits-of-recombinant-antibodies.png)
Anti-mCherry antibody [EPR20579] (ab213511)
Protocols
Datasheets and documents
Certificate of Compliance
References (4)
ab213511 has been referenced in 4 publications.
- Tison KV et al. Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT. G3 (Bethesda) 10:495-504 (2020). PubMed: 31767639
- Lu ZH et al. A New Gorilla Adenoviral Vector with Natural Lung Tropism Avoids Liver Toxicity and Is Amenable to Capsid Engineering and Vector Retargeting. J Virol 94:N/A (2020). PubMed: 32102889
- Williams JL et al. Conditional Synaptic Vesicle Markers for Drosophila. G3 (Bethesda) 9:737-748 (2019). PubMed: 30635441
- Flores BN et al. An Intramolecular Salt Bridge Linking TDP43 RNA Binding, Protein Stability, and TDP43-Dependent Neurodegeneration. Cell Rep 27:1133-1150.e8 (2019). PubMed: 31018129
