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  1. Link

    mcherry-antibody-epr20579-ab213511.pdf

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RecombinantRabMAb

Recombinant Anti-mCherry antibody [EPR20579] (ab213511)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (5) Submit a question References (12)

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Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
  • Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
  • Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)
  • Anti-mCherry antibody [EPR20579] (ab213511)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20579] to mCherry
  • Suitable for: IP, WB
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-mCherry antibody [EPR20579]
    See all mCherry primary antibodies
  • Description

    Rabbit monoclonal [EPR20579] to mCherry
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T cells transfected with a His-tagged mCherry whole cell lysate. IP: 293T cells transfected with a His-tagged mCherry whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20579
  • Isotype

    IgG
  • Research areas

    • Kits/ Lysates/ Other
    • Fluorescent ligands

Associated products

  • Alternative Versions

    • Anti-mCherry antibody [EPR20579] - BSA and Azide free (ab232341)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant mCherry protein (His tag) (ab199750)
  • Related Products

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • mCherry ELISA Kit (ab221829)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (2)
1/20.
WB (2)
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa).
Notes
IP
1/20.
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa).
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Relevance

    mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Images

  • Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    All lanes : Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution

    Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty expression vector whole cell lysate
    Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 29 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    Western blot - Anti-mCherry antibody [EPR20579] (ab213511)
    Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution + Green fluorescent protein (GFP) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Green fluorescent protein (GFP) contains aa1-238 with His-tag.

  • Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)
    Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)

    mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1:  293T transfected with a His-tagged mCherry whole cell lysate 10 µg (Input). 

    Lane 2: ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-mCherry antibody [EPR20579] (ab213511)
    Anti-mCherry antibody [EPR20579] (ab213511)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (12)

Publishing research using ab213511? Please let us know so that we can cite the reference in this datasheet.

ab213511 has been referenced in 12 publications.

  • Toufektchan E & Maciejowski J Purification of micronuclei from cultured cells by flow cytometry. STAR Protoc 2:100378 (2021). PubMed: 33778777
  • Reuwsaat JCV  et al. The Transcription Factor Pdr802 Regulates Titan Cell Formation and Pathogenicity of Cryptococcus neoformans. mBio 12:N/A (2021). PubMed: 33688010
  • Deffains M  et al. In vivo electrophysiological validation of DREADD-based modulation of pallidal neurons in the non-human primate. Eur J Neurosci 53:2192-2204 (2021). PubMed: 32306446
  • Harwalkar K  et al. Anatomical and cellular heterogeneity in the mouse oviduct-its potential roles in reproduction and preimplantation development†. Biol Reprod 104:1249-1261 (2021). PubMed: 33693543
  • McKinney HM  et al. Characterization of Drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines. J Comp Neurol 528:2174-2194 (2020). PubMed: 32060912
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Immunoprecipitation abreview for Anti-mCherry antibody [EPR20579]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEF cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
MEF cells
Read More

Abcam user community

Verified customer

Submitted Mar 04 2019

Immunoprecipitation abreview for Anti-mCherry antibody [EPR20579]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
293T cells
Read More

Abcam user community

Verified customer

Submitted Feb 12 2019

Western blot abreview for Anti-mCherry antibody [EPR20579]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 24 2019

Western blot abreview for Anti-mCherry antibody [EPR20579]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
293T cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 16 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-mCherry antibody [EPR20579]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Tumor Cells Engineered to Express mCherry)
Permeabilization
No
Specification
Tumor Cells Engineered to Express mCherry
Blocking step
10% Donkey Serum in 3% BSA/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Fixative
Paraformaldehyde
Read More

DR. Francesco Elia Marino

Verified customer

Submitted Sep 21 2018

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