Recombinant Anti-mCherry antibody [EPR20579] (ab213511)
![Western blot - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280627-anti-mcherry-antibody-epr20579-western-blot.jpg "Western blot - Anti-mCherry antibody [EPR20579] (ab213511)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20579] to mCherry
- Suitable for: IP, WB
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-mCherry antibody [EPR20579]
See all mCherry primary antibodies -
Description
Rabbit monoclonal [EPR20579] to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T cells transfected with a His-tagged mCherry whole cell lysate. IP: 293T cells transfected with a His-tagged mCherry whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20579 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | (2) |
1/20.
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| WB | (2) |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa).
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| Notes |
|---|
|
IP
1/20. |
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WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 26 kDa). |
Target
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Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
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Western blot - Anti-mCherry antibody [EPR20579] (ab213511)All lanes : Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution
Lane 1 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty expression vector whole cell lysate
Lane 2 : 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280626-anti-mcherry-antibody-epr20579-western-blot.jpg)
Western blot - Anti-mCherry antibody [EPR20579] (ab213511)Anti-mCherry antibody [EPR20579] (ab213511) at 1/1000 dilution + Green fluorescent protein (GFP) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Green fluorescent protein (GFP) contains aa1-238 with His-tag.
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![Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-280620-anti-mcherry-antibody-epr20579-immunoprecipitation.jpg)
Immunoprecipitation - Anti-mCherry antibody [EPR20579] (ab213511)mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: 293T transfected with a His-tagged mCherry whole cell lysate 10 µg (Input).
Lane 2: ab213511 IP in 293T transfected with a His-tagged mCherry whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213511 in 293T transfected with a His-tagged mCherry whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-mCherry antibody [EPR20579] (ab213511)](https://www.abcam.com/ps/products/213/ab213511/Images/ab213511-5-benefits-of-recombinant-antibodies.png)
Anti-mCherry antibody [EPR20579] (ab213511)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (12)
ab213511 has been referenced in 12 publications.
- Toufektchan E & Maciejowski J Purification of micronuclei from cultured cells by flow cytometry. STAR Protoc 2:100378 (2021). PubMed: 33778777
- Reuwsaat JCV et al. The Transcription Factor Pdr802 Regulates Titan Cell Formation and Pathogenicity of Cryptococcus neoformans. mBio 12:N/A (2021). PubMed: 33688010
- Deffains M et al. In vivo electrophysiological validation of DREADD-based modulation of pallidal neurons in the non-human primate. Eur J Neurosci 53:2192-2204 (2021). PubMed: 32306446
- Harwalkar K et al. Anatomical and cellular heterogeneity in the mouse oviduct-its potential roles in reproduction and preimplantation development†. Biol Reprod 104:1249-1261 (2021). PubMed: 33693543
- McKinney HM et al. Characterization of Drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines. J Comp Neurol 528:2174-2194 (2020). PubMed: 32060912