Overview

  • Product name

    Anti-MCJ antibody [EPR12823]
    See all MCJ primary antibodies
  • Description

    Rabbit monoclonal [EPR12823] to MCJ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human MCJ. The exact sequence is proprietary.
    Database link: Q9Y5T4

  • Positive control

    • MCF-7, HepG2, Jurkat and fetal brain lysates; Human lung carcinoma and testis tissues
  • General notes

    Protein previously labeled as DNAJC15.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.
    • Tissue specificity

      Expressed at highest levels in heart, followed by liver and kidney.
    • Involvement in disease

      Absent or down-regulated in many advanced cases of ovarian adenocarcinoma, due to hypermethylation and allelic loss. Loss of expression correlates with increased resistance to antineoplastic drugs, such as cisplatin.
    • Sequence similarities

      Contains 1 J domain.
    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell growth inhibiting gene 22 protein antibody
      • Cell growth-inhibiting gene 22 protein antibody
      • DJC15_HUMAN antibody
      • DnaJ (Hsp40) homolog subfamily C member 15 antibody
      • DNAJ domain containing antibody
      • DnaJ homolog subfamily C member 15 antibody
      • DNAJC15 antibody
      • DNAJD1 antibody
      • GIG22 antibody
      • HSD18 antibody
      • MCJ antibody
      • Methylation controlled J protein antibody
      • Methylation-controlled J protein antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MCJ using ab179820 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling MCJ using ab179820 at 1/50 dilution.

    • All lanes : Anti-MCJ antibody [EPR12823] (ab179820) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 16 kDa

    References

    ab179820 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab179820.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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