Overview

  • Product name

  • Description

    Rabbit polyclonal to MCK10/NEP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MCK10/NEP (internal sequence). The exact sequence is proprietary.
    Database link: Q08345

  • Positive control

    • IHC-P: Human ovarian cancer tissue. WB: Mouse brain and H1299 whole cell lysate.
  • General notes

     This product was previously labelled as MCK10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/100000. Predicted molecular weight: 101 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May be involved in cell-cell interactions and recognition.
  • Tissue specificity

    Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • Domain

    The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
  • Cellular localization

    Secreted and Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAK antibody
    • CD 167 antibody
    • CD167 antibody
    • CD167 antigen-like family member A antibody
    • CD167a antibody
    • Cell adhesion kinase antibody
    • DDR 1 antibody
    • DDR antibody
    • DDR1 antibody
    • DDR1_HUMAN antibody
    • Discoidin domain receptor antibody
    • Discoidin domain receptor tyrosine kinase 1 antibody
    • Discoidin receptor tyrosine kinase antibody
    • Discoidin receptor tyrosine kinase isoform a antibody
    • EDDR 1 antibody
    • EDDR1 antibody
    • Epithelial discoidin domain receptor 1 antibody
    • Epithelial discoidin domain-containing receptor 1 antibody
    • Epithelial specific receptor kinase antibody
    • HGK2 antibody
    • Mammarian carcinoma kinase 10 antibody
    • Mammary carcinoma kinase 10 antibody
    • MCK-10 antibody
    • MCK10 antibody
    • NEP antibody
    • Neuroepithelial tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor type 4 antibody
    • NTRK 4 antibody
    • NTRK4 antibody
    • OTTHUMP00000029343 antibody
    • OTTHUMP00000029344 antibody
    • OTTHUMP00000029345 antibody
    • OTTHUMP00000029346 antibody
    • OTTHUMP00000029347 antibody
    • OTTHUMP00000164863 antibody
    • OTTHUMP00000164867 antibody
    • OTTHUMP00000222080 antibody
    • Protein-tyrosine kinase 3A antibody
    • Protein-tyrosine kinase RTK-6 antibody
    • PTK 3 antibody
    • PTK 3A protein tyrosine kinase 3A antibody
    • PTK3 antibody
    • PTK3A antibody
    • Receptor tyrosine kinase NEP antibody
    • RTK 6 antibody
    • RTK6 antibody
    • TRK E antibody
    • TRKE antibody
    • Tyrosine kinase DDR antibody
    • Tyrosine kinase receptor E antibody
    • Tyrosine-protein kinase CAK antibody
    see all

Images

  • Paraffin embedded human ovarian cancer tissue stained for MCK10/NEP with ab227195 (1/500 dilution) in immunohistochemical analysis.

  • Anti-MCK10/NEP antibody (ab227195) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    7.5% SDS PAGE

  • Anti-MCK10/NEP antibody (ab227195) at 1/5000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    7.5% SDS PAGE

References

ab227195 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - TritonX-100
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde

Miss. Simona Hankeova

Verified customer

Submitted Jul 17 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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