Overview

  • Product name

    Anti-MCK10/NEP (phospho Y513) antibody
    See all MCK10/NEP primary antibodies
  • Description

    Rabbit polyclonal to MCK10/NEP (phospho Y513)
  • Host species

    Rabbit
  • Specificity

    ab193550 detects endogenous levels of MCK10/NEP only when phosphorylated at tyrosine 513.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MCK10/NEP (phospho Y513) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q08345

  • Positive control

    • Extracts from JK cells. Human brain tissue.
  • General notes

     This product was previously labelled as MCK10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab193550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 101 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    May be involved in cell-cell interactions and recognition.
  • Tissue specificity

    Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.
  • Domain

    The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
  • Cellular localization

    Secreted and Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAK antibody
    • CD 167 antibody
    • CD167 antibody
    • CD167 antigen-like family member A antibody
    • CD167a antibody
    • Cell adhesion kinase antibody
    • DDR 1 antibody
    • DDR antibody
    • DDR1 antibody
    • DDR1_HUMAN antibody
    • Discoidin domain receptor antibody
    • Discoidin domain receptor tyrosine kinase 1 antibody
    • Discoidin receptor tyrosine kinase antibody
    • Discoidin receptor tyrosine kinase isoform a antibody
    • EDDR 1 antibody
    • EDDR1 antibody
    • Epithelial discoidin domain receptor 1 antibody
    • Epithelial discoidin domain-containing receptor 1 antibody
    • Epithelial specific receptor kinase antibody
    • HGK2 antibody
    • Mammarian carcinoma kinase 10 antibody
    • Mammary carcinoma kinase 10 antibody
    • MCK-10 antibody
    • MCK10 antibody
    • NEP antibody
    • Neuroepithelial tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor type 4 antibody
    • NTRK 4 antibody
    • NTRK4 antibody
    • OTTHUMP00000029343 antibody
    • OTTHUMP00000029344 antibody
    • OTTHUMP00000029345 antibody
    • OTTHUMP00000029346 antibody
    • OTTHUMP00000029347 antibody
    • OTTHUMP00000164863 antibody
    • OTTHUMP00000164867 antibody
    • OTTHUMP00000222080 antibody
    • Protein-tyrosine kinase 3A antibody
    • Protein-tyrosine kinase RTK-6 antibody
    • PTK 3 antibody
    • PTK 3A protein tyrosine kinase 3A antibody
    • PTK3 antibody
    • PTK3A antibody
    • Receptor tyrosine kinase NEP antibody
    • RTK 6 antibody
    • RTK6 antibody
    • TRK E antibody
    • TRKE antibody
    • Tyrosine kinase DDR antibody
    • Tyrosine kinase receptor E antibody
    • Tyrosine-protein kinase CAK antibody
    see all

Images

  • All lanes : Anti-MCK10/NEP (phospho Y513) antibody (ab193550) at 1/500 dilution

    Lane 1 : Jurkat cell extract with antigen specific peptide
    Lane 2 : Extracts from JK cells

    Predicted band size: 101 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue sections labeling MCK10/NEP (phospho Y513) with ab193550 at a 1/50 dilution. Right image treated with blocking peptide.

References

ab193550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193550.
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