Key features and details
- Rabbit polyclonal to MCK10/NEP (phospho Y513)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MCK10/NEP (phospho Y513) antibody
See all MCK10/NEP primary antibodies
DescriptionRabbit polyclonal to MCK10/NEP (phospho Y513)
ab193550 detects endogenous levels of MCK10/NEP only when phosphorylated at tyrosine 513.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human MCK10/NEP (phospho Y513) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q08345
- Extracts from JK cells. Human brain tissue.
This product was previously labelled as MCK10
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab193550 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab193550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 101 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionMay be involved in cell-cell interactions and recognition.
Tissue specificityDetected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain.
DomainThe Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
Cellular localizationSecreted and Membrane.
- Information by UniProt
- CAK antibody
- CD 167 antibody
- CD167 antibody
All lanes : Anti-MCK10/NEP (phospho Y513) antibody (ab193550) at 1/500 dilution
Lane 1 : Jurkat cell extract with antigen specific peptide
Lane 2 : Extracts from JK cells
Predicted band size: 101 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue sections labeling MCK10/NEP (phospho Y513) with ab193550 at a 1/50 dilution. Right image treated with blocking peptide.
ab193550 has not yet been referenced specifically in any publications.