- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-MCL1 antibody
See all MCL1 primary antibodies -
DescriptionRabbit polyclonal to MCL1
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Host speciesRabbit
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SpecificityThis antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples.
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Rabbit, Guinea pig, Dog, Human, Pig, Monkey
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Immunogen
Synthetic peptide corresponding to Human MCL1 aa 121-139 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:SPEEELDGYEPEPLGKRPA
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Positive control
- HeLa heat shocked cell lysate IHC-P: FFPE human lung tissue sections.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 8.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab28147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 µg/ml. Predicted molecular weight: 42-43 kDa. 0.1 µg/ml was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis. |
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| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
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Sequence similaritiesBelongs to the Bcl-2 family.
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Post-translational
modificationsCleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. -
Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
- Information by UniProt
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Database links
- Entrez Gene: 403537 Dog
- Entrez Gene: 4170 Human
- Entrez Gene: 100359222 Rabbit
- Omim: 159552 Human
- SwissProt: Q8HYS5 Dog
- SwissProt: Q07820 Human
- Unigene: 632486 Human
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Alternative names
- Bcl 2 related protein EAT/mcl1 antibody
- Bcl-2-like protein 3 antibody
- Bcl-2-related protein EAT/mcl1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody (ab28147)IHC image of MCL1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28147, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-MCL1 antibody (ab28147) -
Immunocytochemistry/ Immunofluorescence - Anti-MCL1 antibody (ab28147)ab28147 staining MCL1 in human Hella cells by Immunocytochemistry/ immunofluorescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCL1 antibody (ab28147)ab28147 at 1/50 dillution staining MCL1 in human breast cancer tissue section by Immunohistochemistry.
Protocols
References
This product has been referenced in:
- Loriot Y et al. Radiosensitization by a novel Bcl-2 and Bcl-XL inhibitor S44563 in small-cell lung cancer. Cell Death Dis 5:e1423 (2014). WB ; Human . Read more (PubMed: 25232677) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"