Key features and details
- Rabbit polyclonal to MCL1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rabbit, Guinea pig, Dog, Human, Pig, Monkey
- Isotype: IgG
Product nameAnti-MCL1 antibody
See all MCL1 primary antibodies
DescriptionRabbit polyclonal to MCL1
SpecificityThis antibody detects an ~42/43 kDa protein doublet, corresponding to the expected molecular mass of Mcl1 on SDS-PAGE immunoblots. The antibody specificity is confirmed by peptide inhibition studies. An additional protein at ~33kDa may also be detected. Other additional proteins may also be seen in some samples.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rabbit, Guinea pig, Dog, Human, Pig, Monkey
- ICC/IF: Human HeLa cells; IHC-P: Human breast cancer tissue; WB: Hela, MDCK and RK-13 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 µg/ml. Predicted molecular weight: 42-43 kDa.
0.1 µg/ml was sufficient for detection of Mcl1 in 20µg of HeLa Heat Shocked Cell Lysate by ECL immunoblot analysis.
|ICC/IF||Use at an assay dependent concentration.|
FunctionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Sequence similaritiesBelongs to the Bcl-2 family.
modificationsCleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Cellular localizationMembrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
- Information by UniProt
- Bcl 2 related protein EAT/mcl1 antibody
- Bcl-2-like protein 3 antibody
- Bcl-2-related protein EAT/mcl1 antibody
All lanes : Anti-MCL1 antibody (ab28147) at 1/500 dilution
Lane 2 : HeLa whole cell lysate
Lane 3 : 3T3 whole cell lysate
Lane 4 : PC-12 whole cell lysate
Lane 5 : MDCK whole cell lysate
Lane 6 : RK-13 whole cell lysate
Predicted band size: 42-43 kDa
ab28147 staining MCL1 in human breast cancer tissue cells by Immunohistochemistry
ab28147 staining MCL1 in human HeLa cells by Immunocytochemistry/ immunofluorescence.
ab28147 has been referenced in 4 publications.
- Rad S M AH et al. Promoter choice: Who should drive the CAR in T cells? PLoS One 15:e0232915 (2020). PubMed: 32706785
- Jin X et al. MicroRNA-105 promotes epithelial-mesenchymal transition of nonsmall lung cancer cells through upregulating Mcl-1. J Cell Biochem 120:5880-5888 (2019). PubMed: 30317672
- Cui Y et al. Upregulation of microRNA-383 inhibits the proliferation, migration and invasion of colon cancer cells. Oncol Lett 15:1184-1190 (2018). PubMed: 29399173
- Loriot Y et al. Radiosensitization by a novel Bcl-2 and Bcl-XL inhibitor S44563 in small-cell lung cancer. Cell Death Dis 5:e1423 (2014). WB ; Human . PubMed: 25232677