Overview

  • Product name

    Anti-MCL1 (phospho S159) antibody
    See all MCL1 primary antibodies
  • Description

    Rabbit polyclonal to MCL1 (phospho S159)
  • Host species

    Rabbit
  • Specificity

    ab111574 detects endogenous levels of MCL1 only when phosphorylated at serine 159.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from Human MCL1 around the phosphorylation site of Serine 159 (D-G-SP-L-P).

  • Positive control

    • A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab111574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Post-translational
    modifications

    Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
    Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
    Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
  • Cellular localization

    Membrane. Cytoplasm. Mitochondrion. Nucleus > nucleoplasm. Cytoplasmic, associated with mitochondria.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bcl 2 related protein EAT/mcl1 antibody
    • Bcl-2-like protein 3 antibody
    • Bcl-2-related protein EAT/mcl1 antibody
    • BCL2 related antibody
    • Bcl2-L-3 antibody
    • BCL2L3 antibody
    • EAT antibody
    • Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
    • Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
    • MCL 1 antibody
    • MCL1 antibody
    • MCL1-ES antibody
    • mcl1/EAT antibody
    • MCL1_HUMAN antibody
    • MCL1L antibody
    • MCL1S antibody
    • MGC104264 antibody
    • MGC1839 antibody
    • Myeloid Cell Leukemia 1 antibody
    • Myeloid cell leukemia ES antibody
    • Myeloid cell leukemia sequence 1 antibody
    • Myeloid cell leukemia sequence 1 BCL2 related antibody
    • Myeloid cell leukemia sequence 1 isoform 1 antibody
    • OTTHUMP00000032794 antibody
    • OTTHUMP00000032795 antibody
    • TM antibody
    see all

Images

  • ab111574, at 1/100 dilution, staining MCL1 in A549 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.

References

This product has been referenced in:

  • Sarabhai T  et al. Serum a-1 Antitrypsin (AAT) antagonizes intrinsic apoptosis induction in neutrophils from patients with systemic inflammatory response syndrome. PLoS One 12:e0177450 (2017). WB . Read more (PubMed: 28493974) »
  • Weigel KJ  et al. CAF-secreted IGFBPs regulate breast cancer cell anoikis. Mol Cancer Res 12:855-66 (2014). Read more (PubMed: 24803643) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

I have replaced the image with the one you just sent me, and copied the figure legend into the Additional Notes box on the Abreview. The review will be published shortly.

Thank you for testing the antibody in WB and for submitting your review.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Liver cells)
Loading amount
80 µg
Specification
Liver cells
Treatment
DMSO 24 h or tBHQ different time periods
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 05 2012

Question
Answer

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab111574 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Question
Answer

I have confirmed that the antibody ab111574, raised against MCL1 pSer159, has not been tested for reactivity with MCL1 pSer159/pThr163.

Read More

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