Product nameAnti-MCM-BP antibody
See all MCM-BP primary antibodies
DescriptionRabbit polyclonal to MCM-BP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human stomach tissue; RT4, U 251 MG, Human plasma, liver and tonsil lysates; A431s.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.|
FunctionAssociated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
Sequence similaritiesBelongs to the MCMBP family.
Cellular localizationNucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
- Information by UniProt
- C10orf119 antibody
- chromosome 10 open reading frame 119 antibody
- MCM-binding protein antibody
ab122478 at 1/50 dilution staining MCM-BP in paraffin-embedded Human stomach tissue by Immunohistochemistry.
ab122478 at 4 µg/ml staining MCM-BP in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
All lanes : Anti-MCM-BP antibody (ab122478) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
ab122478 has not yet been referenced specifically in any publications.