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    mcm-bp-antibody-ab124290.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-MCM-BP antibody (ab124290)

  • Datasheet
  • SDS
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Western blot - Anti-MCM-BP antibody (ab124290)
  • Immunoprecipitation - Anti-MCM-BP antibody (ab124290)

Key features and details

  • Rabbit polyclonal to MCM-BP
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MCM-BP antibody
    See all MCM-BP primary antibodies
  • Description

    Rabbit polyclonal to MCM-BP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human MCM-BP (NP_079110.1).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab124290 was affinity purified using an epitope specific to MCM-BP immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
  • Sequence similarities

    Belongs to the MCMBP family.
  • Cellular localization

    Nucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
  • Target information above from: UniProt accession Q9BTE3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 79892 Human
    • Omim: 610909 Human
    • SwissProt: Q9BTE3 Human
    • Unigene: 124246 Human
    • Alternative names

      • C10orf119 antibody
      • chromosome 10 open reading frame 119 antibody
      • MCM-binding protein antibody
      • MCM-BP antibody
      • mcmbp antibody
      • MCMBP_HUMAN antibody
      • Mini-chromosome maintenance complex-binding protein antibody
      • Minichromosome maintenance complex binding protein antibody
      see all

    Images

    • Western blot - Anti-MCM-BP antibody (ab124290)
      Western blot - Anti-MCM-BP antibody (ab124290)
      All lanes : Anti-MCM-BP antibody (ab124290) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-MCM-BP antibody (ab124290)
      Immunoprecipitation - Anti-MCM-BP antibody (ab124290)
      ab124290 at 1 µg/ml staining MCM-BP in HeLa whole cell lysate immunoprecipitated using ab124290 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 73 kDa. Exposure time: 30 seconds

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124290? Please let us know so that we can cite the reference in this datasheet.

    ab124290 has not yet been referenced specifically in any publications.

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