Anti-MCM-BP antibody (ab124290)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MCM-BP antibody
See all MCM-BP primary antibodies -
Description
Rabbit polyclonal to MCM-BP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human MCM-BP (NP_079110.1).
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Positive control
- HeLa, 293T and Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab124290 was affinity purified using an epitope specific to MCM-BP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion. -
Sequence similarities
Belongs to the MCMBP family. -
Cellular localization
Nucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex. - Information by UniProt
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Database links
- Entrez Gene: 79892 Human
- Omim: 610909 Human
- SwissProt: Q9BTE3 Human
- Unigene: 124246 Human
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Alternative names
- C10orf119 antibody
- chromosome 10 open reading frame 119 antibody
- MCM-binding protein antibody
see all
Images
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Western blot - Anti-MCM-BP antibody (ab124290)All lanes : Anti-MCM-BP antibody (ab124290) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes -
Immunoprecipitation - Anti-MCM-BP antibody (ab124290)ab124290 at 1 µg/ml staining MCM-BP in HeLa whole cell lysate immunoprecipitated using ab124290 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 73 kDa. Exposure time: 30 seconds
Datasheets and documents
References
ab124290 has not yet been referenced specifically in any publications.
