Anti-MCM-BP antibody - N-terminal (ab174343)

Overview

  • Product name
    Anti-MCM-BP antibody - N-terminal
    See all MCM-BP primary antibodies
  • Description
    Rabbit polyclonal to MCM-BP - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human MCM-BP aa 150-179 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9BTE3

  • Positive control
    • Human cervix carcinoma tissue; MDA-MB231 cells; MDA-MB231 cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174343 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 73 kDa.

Target

  • Function
    Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
  • Sequence similarities
    Belongs to the MCMBP family.
  • Cellular localization
    Nucleus. Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase. Dissociates from chromatin later than component of the MCM complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • C10orf119 antibody
    • chromosome 10 open reading frame 119 antibody
    • MCM-binding protein antibody
    • MCM-BP antibody
    • mcmbp antibody
    • MCMBP_HUMAN antibody
    • Mini-chromosome maintenance complex-binding protein antibody
    • Minichromosome maintenance complex binding protein antibody
    see all

Images

  • Anti-MCM-BP antibody - N-terminal (ab174343) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size: 73 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervix carcinoma tissue labeling MCM-BP with ab174343 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of MDA-MB231 cells labeling MCM-BP with ab174343 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174343 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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