Overview

  • Product name

  • Description

    Rabbit polyclonal to MCM10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, OrangutanDoes not react with: Xenopus laevis, Drosophila melanogaster
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.

  • Positive control

    • HeLa cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

Target

  • Function

    Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.
  • Sequence similarities

    Belongs to the MCM10 family.
  • Developmental stage

    Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.
  • Domain

    The zinc finger-like domain binds a zinc ion and is involved in self-association.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
  • Information by UniProt
  • Database links

  • Alternative names

    • CNA43 antibody
    • DNA43 antibody
    • FLJ31133 antibody
    • Homolog of yeast MCM10 antibody
    • HsMCM10 antibody
    • mcm10 antibody
    • MCM10 minichromosome maintenance deficient 10 antibody
    • MCM10_HUMAN antibody
    • Minichromosome maintenance 10 replication initiation factor antibody
    • Minichromosome maintenance complex component 10 antibody
    • Minichromosome maintenance protein 10 isoform 1 antibody
    • PRO2249 antibody
    • Protein MCM10 homolog antibody
    see all

Images

  • Sample: Hela cell lysate (1mg for IP).

    Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.

    Detection: ECL.

     

    Sample: Hela cell lysate (1mg for IP).

    Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.

    Detection: ECL.

References

This product has been referenced in:

  • Ma Y  et al. Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Oncotarget 8:104813-104830 (2017). Read more (PubMed: 29285216) »
  • Liao YX  et al. Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells. Mol Med Rep 13:4872-8 (2016). Read more (PubMed: 27082503) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells and BJ human fibroblast cells)
Loading amount
20 µg
Specification
HeLa cells and BJ human fibroblast cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 03 2008

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