Anti-MCM2 antibody (ab4461)
Key features and details
- Rabbit polyclonal to MCM2
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MCM2 antibody
See all MCM2 primary antibodies -
Description
Rabbit polyclonal to MCM2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human MCM2. The epitope recognized maps to a region between residues 1 and 50 of human human minichromosomal maintenance deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171).
Database link: P49736
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Chimpanzee
Gorilla
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP | (2) |
Use at 2-5 µg/mg of lysate.
|
| WB | (14) |
1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
|
| Notes |
|---|
|
IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 100 kDa. |
Target
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Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain. -
Post-translational
modificationsPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Images
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Western blot - Anti-MCM2 antibody (ab4461)All lanes : Anti-MCM2 antibody (ab4461) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody (ab4461)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labeling MCM2 with ab4461 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-MCM2 antibody (ab4461)MCM2 was immunoprecipitated from 1 mg per reaction of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab4461 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab4461 at 1 µg/ml.
Lane 1: Phospho MCM2 (S108) antibody IP in HEK-293T lysate.
Lane 2: ab4461 IP in HEK-293T lysate.
Lane 3: Control IgG instead of ab4461 in HEK-293T lysate.
NETN lysis buffer.
Exposure time: XXXX. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody (ab4461)Ab4461 staining MCM2 in Human placenta.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References (35)
ab4461 has been referenced in 35 publications.
- Lovejoy CA et al. ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biol 18:e3000594 (2020). PubMed: 31895940
- Lai Y & Yang Y SMYD2 facilitates cancer cell malignancy and xenograft tumor development through ERBB2-mediated FUT4 expression in colon cancer. Mol Cell Biochem N/A:N/A (2020). PubMed: 32342276
- Coolen M et al. Mosaic Heterochrony in Neural Progenitors Sustains Accelerated Brain Growth and Neurogenesis in the Juvenile Killifish N. furzeri. Curr Biol 30:736-745.e4 (2020). PubMed: 32004451
- Fan Q et al. The intracellular domain of CX3CL1 regulates adult neurogenesis and Alzheimer's amyloid pathology. J Exp Med 216:1891-1903 (2019). PubMed: 31209068
- Wang C et al. Inducing and exploiting vulnerabilities for the treatment of liver cancer. Nature 574:268-272 (2019). PubMed: 31578521
